Anti-AMSH 抗体 [EPR4361]

• WB

Lab

Western blot - Anti-AMSH antibody [EPR4361] (AB108301)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE. ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

AMSH knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Jurkat whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Predicted band size: 48 kDa

false

• WB

Lab

Western blot - Anti-AMSH antibody [EPR4361] (AB108301)

ab108301 Anti-STAMBP antibody was shown to specifically react with STAM Binding Protein in HeLa wild-type cells (ab255928). Loss of signal was observed when knockout cell line ab265249 (knockout cell lysate ab258213) was used. Wild-type and STAM Binding Protein knockout samples were subjected to SDS-PAGE. ab108301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

STAM Binding Protein knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human STAMBP (AMSH) knockout HeLa cell line (<a href='/products/cell-lines/human-stambp-amsh-knockout-hela-cell-line-ab265249'>ab265249</a>)

Lane 3:

HAP1 lysate at 20 µg

Lane 4:

Jurkat lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

• WB

Unknown

Western blot - Anti-AMSH antibody [EPR4361] (AB108301)

All lanes:

Western blot - Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

SW480 cell lysate at 10 µg

Predicted band size: 48 kDa

false