AB68206

Anti-AMPK beta 1 (phospho S181) 抗体 [EP2181Y]

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal AMPK beta 1 phospho S181 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

別名を表示する

AMPK, PRKAB1, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb

3 Images

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

293T whole cell lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 20s

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

Rat heart lysate at 15 µg

Lane 2:

Rat heart lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Mouse brain lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2181Y

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse, Rat

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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製品の詳細

This product has switched from a hybridoma to recombinant production method on 7th February 2025.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

See full target information PRKAB1 pS181

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw1059 PubMed40864710

2025

genotype influences androgen response in developing murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly N Roeszler,Michael See,Lyra R Meehan,Giscard Lima,Alexander Kolliari-Turner,Sarah E Alexander,Shanie Landen,Harrison D Wood,Chrystal F Tiong,Weiyi Chen,Tomris Mustafa,Peter J Houweling,Nir Eynon,Severine Lamon,Yannis Pitsiladis,David J Handelsman,Fernando J Rossello,Mirana Ramialison,Kathryn N North,Jane T Seto

Journal of cellular and molecular medicine 25:8174-8186 PubMed34309216

2021

LOC100996425 acts as a promoter in prostate cancer by mediating hepatocyte nuclear factor 4A and the AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyan Wang,Bin Song,Mingcui Zang,He Ji,He Yang,Shuang Jiang,Xiao Yang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPK beta 1 (phospho S181) 抗体 [EP2181Y]

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise