Anti-Amphiregulin 抗体 (ab180722)
Key features and details
- Rabbit polyclonal to Amphiregulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Amphiregulin antibody
Amphiregulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Amphiregulin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human Amphiregulin aa 20-252. Mature Protein without signal peptide.
Database link: P15514 -
ポジティブ･コントロール
- WB: MCF7, A549, HT29 and Mouse brain lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180722の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 28 kDa.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 28 kDa. |
ターゲット情報
-
機能
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
配列類似性
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 374 Human
- Entrez Gene: 727738 Human
- Entrez Gene: 11839 Mouse
- Omim: 104640 Human
- SwissProt: P15514 Human
- SwissProt: P31955 Mouse
- Unigene: 270833 Human
- Unigene: 645475 Human
see all -
別名
- A REG antibody
- Amphiregulin antibody
- Amphiregulin B antibody
see all
画像
-
Western blot - Anti-Amphiregulin antibody (ab180722)Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
-
Western blot - Anti-Amphiregulin antibody (ab180722)Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + Mouse brain lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 300 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
-
Western blot - Anti-Amphiregulin antibody (ab180722)All lanes : Anti-Amphiregulin antibody (ab180722) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : A549 cell extracts
Lane 3 : HT29 cell extracts
Predicted band size: 28 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab180722 は 3 報の論文で使用されています。
- Geng H et al. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34396425
- Chen S et al. Renal subcapsular delivery of PGE2 promotes kidney repair by activating endogenous Sox9+ stem cells. iScience 24:103243 (2021). PubMed: 34746706
- Dong X et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166