Anti-Amphiphysin 抗体 [EP2060Y] (ab52646)
![Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.co.jp/ps/products/52/ab52646/Images/ab52646-65072-anti-amphiphysin-antibody-ep2060y-western-blot.jpg "Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2060Y] to Amphiphysin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Amphiphysin antibody [EP2060Y]
Amphiphysin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2060Y] to Amphiphysin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human brain lysate. IHC-P: Human brain tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2060Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52646の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. -
組織特異性
Neurons, certain endocrine cell types and spermatocytes. -
配列類似性
Contains 1 BAR domain.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 273 Human
- Entrez Gene: 218038 Mouse
- Entrez Gene: 60668 Rat
- Omim: 600418 Human
- SwissProt: P49418 Human
- SwissProt: Q7TQF7 Mouse
- SwissProt: O08838 Rat
- Unigene: 592182 Human
see all -
別名
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
see all
画像
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Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.co.jp/ps/products/52/ab52646/Images/ab52646-65071-anti-amphiphysin-antibody-ep2060y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2060Y] (ab52646)Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.co.jp/ps/products/52/ab52646/Images/ab52646-5-benefits-of-recombinant-antibodies.png)
Anti-Amphiphysin antibody [EP2060Y] (ab52646)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab52646 は 3 報の論文で使用されています。
- Buser DP et al. Quantitative proteomics reveals reduction of endocytic machinery components in gliomas. EBioMedicine 46:32-41 (2019). PubMed: 31331834
- Bosch PJ et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . PubMed: 26484527
- Kumar GS et al. Quantitative proteomics for identifying biomarkers for tuberculous meningitis. Clin Proteomics 9:12 (2012). IHC . PubMed: 23198679