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リコンビナント

Anti-AMID 抗体 [6D8] (ab302673)

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Western blot - Anti-AMID antibody [6D8] (AB302673)
  • Immunoprecipitation - Anti-AMID antibody [6D8] (AB302673)
  • Anti-AMID antibody [6D8] (AB302673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6D8] to AMID
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AMID antibody [6D8]
    AMID 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [6D8] to AMID

  • 由来種

    Rat

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A375, HepG2, A549, T47D, MCF7 whole cell lysates. IP: HepG2 whole cell lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Ion Exchange Chromatography

  • ポリ/モノ

    モノクローナル

  • クローン名

    6D8

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302673の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能

    Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest.

  • 組織特異性

    Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells.

  • 配列類似性

    Belongs to the FAD-dependent oxidoreductase family.

  • 細胞内局在

    Cytoplasm. Mitochondrion outer membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 5430437E11Rik antibody
      • aifm2 antibody
      • AIFM2_HUMAN antibody
      • AMID antibody
      • Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody
      • Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody
      • Apoptosis inducing factor mitochondrion associated 2 antibody
      • Apoptosis-inducing factor 2 antibody
      • Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody
      • Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody
      • Cys51Stop antibody
      • Ferroptosis suppressor protein 1 antibody
      • FSP1 antibody
      • HGNC11998 antibody
      • p53 responsive gene 3 antibody
      • p53 tumor suppressor antibody
      • p53-responsive gene 3 protein antibody
      • PRG3 antibody
      • TRP53 antibody
      • Tumor protein p53 antibody
      see all

    画像

    • Western blot - Anti-AMID antibody [6D8] (AB302673)
      Western blot - Anti-AMID antibody [6D8] (AB302673)
      All lanes : Anti-AMID antibody [6D8] (ab302673) at 1/1000 dilution

      Lane 1 : A375 (human malignant melanoma epithelial cell), whole cell lysate
      Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 4 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
      Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

      Predicted band size: 41 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    • Immunoprecipitation - Anti-AMID antibody [6D8] (AB302673)
      Immunoprecipitation - Anti-AMID antibody [6D8] (AB302673)

      AMID was immunoprecipitated from 10μg of HepG2 whole cell lysate with ab302673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab302673 at 1/1000 dilution. ab205720 (Goat Anti-Rat IgG (H+L), HRP)) was used as secondary antibody at 1/10000 dilution.

      Lane 1 (Input): HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 μg.

      Lane 2 (+): HepG2 whole cell lysate 10 μg.

      Lane 3 (-): Rat IgG2a, kappa monoclonal isotype Control (ab18450) instead of ab302674 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      Observed MW(KDa): 40

    • Anti-AMID antibody [6D8] (AB302673)
      Anti-AMID antibody [6D8] (AB302673)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302673 は論文での使用が確認できていません。

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