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Anti-AMH 抗体 (ab84415)

Submit a review Q&A (4)References (1)
Western blot - Anti-AMH antibody (ab84415)

    Key features and details

    • Rabbit polyclonal to AMH
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-AMH antibody
      AMH 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to AMH

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab84415の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/200 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
    特記事項
    WB
    1/200 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

    ターゲット情報

    • 関連性

      Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.

    • 細胞内局在

      Secreted

    • 参照データベース

      • 別名

        • Anti muellerian hormone antibody
        • MIF antibody
        • MIS antibody
        • Muellerian inhibiting factor antibody
        • Mullerian inhibiting substance antibody
        see all

      画像

      • Western blot - Anti-AMH antibody (ab84415)
        Western blot - Anti-AMH antibody (ab84415)
        Anti-AMH antibody (ab84415) at 1/1000 dilution + human fetal testis lysates

        Predicted band size: 60 kDa
        Observed band size: 60 kDa

      参考文献 (1)

      ab84415 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab84415 は 1 報の論文で使用されています。

      • Sepponen K  et al. Steroidogenic factor 1 (NR5A1) induces multiple transcriptional changes during differentiation of human gonadal-like cells. Differentiation 128:83-100 (2022). PubMed: 36114074

      レビューと Q&A

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      レビューを送る 質問を送る

      1-4 of 4 Abreviews or Q&A

      Question

      Dear Sir,
      I have purchased Abcam antibody on 25.01.2012.Upon receipt the antibody was stored at -80.I want toknow the expiry of the antibody.I want to give a buffer

      exchange for that antibody and after that how long it can be used? Please reply to me at the earliest.

      Best,

      Read More
      Answer

      Thank you for contacting us.

      We generally recommend an expiry date of 10 years for antibodies stored at -80C. 50% Glycerol is recommended when abs stored at -80C.

      We would not be able to guarantee the antibody after buffer exchange. Theatrically, it should be fine however we will not be able ot confirm it due to lack of tested data.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Can you please let me know the concentration of ab84415 as it is not clear from the data sheet.

      Read More
      Answer

      Thank you for contacting us. The concentration of the current lot of this Protein A purified anti-AMH antibody is 10mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Do you have any information about these AMH antibodies having been tested in a paired assay (sandwich ELISA, competetive ELISA, etc)?

      Read More
      Answer

      Thank you for contacting us.  I have confirmed with the labs that made ab84415 and ab90241 that they have no information about anyone having ever tested these antibodies in paired applications.  I am sorry I could not provide any specific recommendations, please let me know if you have any additional questions or concerns. 

      Read More

      Question

      What is the immunogen? Epitope binding?

      Read More
      Answer

      Thank you for your enquiry. ab84415 - The immunogen is a 15 amino acid peptide within the 50 amino acids of the C terminus of human AMH/MIS: LKMQVRGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR The exact sequence is considered to be proprietary information. The epitopes have not been mapped but they will be within that 50 aa range. ab90241 - The immunogen is a recombinant fragment of human AMH/MIS, within the sequence of amino acids 455 - 560, protein access number # NP_000470. The epitope has not, to our knowledge, been mapped.

      Read More

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