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Anti-Aly/Ref 抗体 [11G5] (ab6141)

Reviews (1)Q&A (1)References (20)
Immunocytochemistry/ Immunofluorescence - Anti-Aly/Ref antibody [11G5] (ab6141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)
  • Flow Cytometry - Anti-Aly/Ref antibody [11G5] (ab6141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)

Key features and details

  • Mouse monoclonal [11G5] to Aly/Ref
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Aly/Ref antibody [11G5]
    Aly/Ref 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [11G5] to Aly/Ref

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Aly/Ref.
    Database link: Q86V81

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified from supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    11G5

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6141の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. THOC4 in conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA.
    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.

  • 配列類似性

    Belongs to the THOC4 family.
    Contains 1 RRM (RNA recognition motif) domain.

  • 翻訳後修飾

    Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Nucleus speckle. Cytoplasm. Colocalizes with the core EJC, THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Ally of AML-1 and LEF-1 antibody
      • Ally of AML1 and LEF1 antibody
      • ALY antibody
      • ALY/REF antibody
      • Aly/REF export factor antibody
      • BEF antibody
      • bZIP enhancing factor antibody
      • bZIP-enhancing factor BEF antibody
      • REF antibody
      • THO complex 4 antibody
      • THO complex subunit 4 antibody
      • Tho4 antibody
      • thoc4 antibody
      • THOC4_HUMAN antibody
      • Transcriptional coactivator Aly/REF antibody
      • Transcriptional coactivator antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Aly/Ref antibody [11G5] (ab6141)
      Immunocytochemistry/ Immunofluorescence - Anti-Aly/Ref antibody [11G5] (ab6141)
      ICC/IF image of ab6141 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6141, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)
      IHC image of ab6141 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6141, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-Aly/Ref antibody [11G5] (ab6141)
      Flow Cytometry - Anti-Aly/Ref antibody [11G5] (ab6141)
      Overlay histogram showing HeLa cells stained with ab6141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6141, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)

      Immunohistochemistry showing Aly/Ref in formalin fixed paraffin embedded skin tissue using ab6141 at 1 μg/ml.

    参考文献 (20)

    ab6141 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6141 は 20 報の論文で使用されています。

    • Tan X  et al. Identification and verification of an ALYREF-involved 5-methylcytosine based signature for stratification of prostate cancer patients and prediction of clinical outcome and response to therapies. Discov Oncol 14:62 (2023). PubMed: 37155024
    • LaClair KD  et al. Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS. Acta Neuropathol 140:121-142 (2020). PubMed: 32562018
    • Noto PB  et al. Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues. Sci Rep 10:22155 (2020). PubMed: 33335114
    • Berson A  et al. Drosophila Ref1/ALYREF regulates transcription and toxicity associated with ALS/FTD disease etiologies. Acta Neuropathol Commun 7:65 (2019). PubMed: 31036086
    • Lesbirel S  et al. The m6A-methylase complex recruits TREX and regulates mRNA export. Sci Rep 8:13827 (2018). PubMed: 30218090
    View all Publications for this product

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Aly/Ref antibody [11G5]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    HCT116
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 30 2016

    Question

    I would like to use an antibody against Aly, preferably the [11G5] antibody, for immunofluorescence staining in HeLa cells. I would like to know what fixation procedure you have used and what working range of dilutions you have used for this antibody. Thanks

    Read More
    Answer

    Thank you for your enquiry. Unfortunately, the originator will not give me any more information regarding the use of this antibody in IF. I have removed this application from the datasheet since I cannot obtain any further information. I apologize for the inconvenience. Please do not hesitate to contact us again if you need anything further.

    Read More

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