Anti-ALX3 抗体 (ab64985)
Key features and details
- Rabbit polyclonal to ALX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALX3 antibody -
製品の詳細
Rabbit polyclonal to ALX3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ALX3 aa 150-250 (internal sequence).
Database link: O95076 -
ポジティブ･コントロール
- 293 cell extract and HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64985の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 257 Human
- Entrez Gene: 11694 Mouse
- Entrez Gene: 365900 Rat
- Omim: 606014 Human
- SwissProt: O95076 Human
- SwissProt: O70137 Mouse
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別名
- ALX 3 antibody
- ALX3 homeobox 3 antibody
- Aristaless like homeobox 3 antibody
see all
画像
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Western blot - Anti-ALX3 antibody (ab64985)All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-ALX3 antibody (ab64985)Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab64985 は 4 報の論文で使用されています。
- Qi J et al. Oncogenic role of ALX3 in cervical cancer cells through KDM2B-mediated histone demethylation of CDC25A. BMC Cancer 21:819 (2021). PubMed: 34266408
- He L et al. Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix. Nat Mater 18:627-637 (2019). PubMed: 31114073
- García-Sanz P et al. Embryonic defence mechanisms against glucose-dependent oxidative stress require enhanced expression of Alx3 to prevent malformations during diabetic pregnancy. Sci Rep 7:389 (2017). PubMed: 28341857
- Mirasierra M & Vallejo M Glucose-dependent downregulation of glucagon gene expression mediated by selective interactions between ALX3 and PAX6 in mouse alpha cells. Diabetologia 59:766-75 (2016). PubMed: 26739814