Anti-ALS2CL 抗体 (ab187986)
Key features and details
- Rabbit polyclonal to ALS2CL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ALS2CL antibody
ALS2CL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ALS2CL -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
-
免疫原
Recombinant fragment corresponding to Human ALS2CL aa 800-950.
Database link: Q60I27 -
ポジティブ･コントロール
- U-251MG sp cell lysate; Human breast tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187986の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 108 kDa.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 108 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosome dynamics. -
組織特異性
Expressed in heart and kidney. -
配列類似性
Contains 8 MORN repeats.
Contains 1 VPS9 domain. -
細胞内局在
Cytoplasm. Distributed onto the vesicular compartments in the cytoplasm with strong punctated staining. Colocalizes with RAB5A onto the vesicular/membranous compartments in the cytoplasm, particularly to the leading edges of the cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 506969 Cow
- Entrez Gene: 259173 Human
- Entrez Gene: 235633 Mouse
- Entrez Gene: 316017 Rat
- Omim: 612402 Human
- SwissProt: A6QP75 Cow
- SwissProt: Q60I27 Human
- SwissProt: Q60I26 Mouse
see all -
別名
- AL2CL_HUMAN antibody
- ALS2 C-terminal like antibody
- ALS2 C-terminal-like protein antibody
see all
画像
-
Western blot - Anti-ALS2CL antibody (ab187986)All lanes : Anti-ALS2CL antibody (ab187986) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251MG sp cell lysate
Predicted band size: 108 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALS2CL antibody (ab187986)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling ALS2CL with ab187986 at 1/50 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab187986 は論文での使用が確認できていません。