Anti-Als2 抗体 [EPR11185] (ab170896)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)](https://www.abcam.co.jp/ps/products/170/ab170896/Images/ab170896-170822-anti-als2-antibody-epr11185-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11185] to Als2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Als2 antibody [EPR11185]
Als2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11185] to Als2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt (Intra),ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Als2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96Q42 -
ポジティブ･コントロール
- SH-SY5Y, 293T and HeLa cell lysates; Human brain and pancreas tissues; SH-SY5Y cells; 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11185 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170896の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 184 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 184 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.
ターゲット情報
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機能
May act as a GTPase regulator. Controls survival and growth of spinal motoneurons. -
関連疾患
Defects in ALS2 are the cause of amyotrophic lateral sclerosis type 2 (ALS2) [MIM:205100]. ALS2 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Defects in ALS2 are the cause of juvenile primary lateral sclerosis (JPLS) [MIM:606353]. JPLS is a neurodegenerative disorder which is closely related to but clinically distinct from amyotrophic lateral sclerosis. It is a progressive paralytic disorder which results from dysfunction of the upper motor neurons of the motor cortex while the lower neurons are unaffected.
Defects in ALS2 are the cause of infantile-onset ascending spastic paralysis (IAHSP) [MIM:607225]. IAHSP is characterized by progressive spasticity and weakness of limbs. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 8 MORN repeats.
Contains 1 PH domain.
Contains 5 RCC1 repeats.
Contains 1 VPS9 domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 57679 Human
- GenBank: NM_020919 Human
- Omim: 606352 Human
- SwissProt: Q96Q42 Human
- Unigene: 471096 Human
- Unigene: 621812 Human
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別名
- ALS 2 antibody
- ALS2 antibody
- ALS2_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Als2 with ab170896 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)](https://www.abcam.co.jp/ps/products/170/ab170896/Images/ab170896-170821-anti-als2-antibody-epr11185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Als2 with ab170896 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Als2 antibody [EPR11185] (ab170896)](https://www.abcam.co.jp/ps/products/170/ab170896/Images/ab170896-170820-anti-als2-antibody-epr11185-western-blot.jpg)
Western blot - Anti-Als2 antibody [EPR11185] (ab170896)All lanes : Anti-Als2 antibody [EPR11185] (ab170896) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 184 kDa -
![Anti-Als2 antibody [EPR11185] (ab170896)](https://www.abcam.co.jp/ps/products/170/ab170896/Images/ab170896-6-benefits-of-recombinant-antibodies.png)
Anti-Als2 antibody [EPR11185] (ab170896)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab170896 は 3 報の論文で使用されています。
- Rivas S et al. The RabGEF ALS2 is a hypoxia inducible target associated with the acquisition of aggressive traits in tumor cells. Sci Rep 10:22302 (2020). PubMed: 33339852
- Gillingham AK et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692
- Arriagada C et al. Focal adhesion kinase-dependent activation of the early endocytic protein Rab5 is associated with cell migration. J Biol Chem 294:12836-12845 (2019). PubMed: 31292193