AB236626

Anti-ALPK1 抗体 [EPR20911-169]

Anti-ALPK1 antibody [EPR20911-169]

20ul selling size
RabMAb
Recombinant
KO Validated
詳細を見る

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(3 Publications)

Rabbit Recombinant Monoclonal ALPK1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

KIAA1527, LAK, ALPK1, Alpha-protein kinase 1, Chromosome 4 kinase, Lymphocyte alpha-protein kinase

2 Images

Collaborator

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.

Exposure time : 5 minutes

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution

Lane 1:

ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

Wild-type 293T whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Lab

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

Blocking and Diluting buffer : 5% NFDM /TBST.

To get satisfied result, the following protocol tips are strongly recommended. Scrap the cells from cell culture flasks with SDS loading buffer and boil them for 10 minutes immediately. Then prepare membranes with this freshly made lysate. Incubate the membrane with ab236626 at the dilution of 1/500 for 3 hours at room temperature and then secondary antibody ab97051 at the dilution of 1/20000 overnight. Acquire WB image with ECL substrate of high sensitivity.

All lanes:

Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution

Lane 1:

Fresh 293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Fresh A549 (Human lung carcinoma epithelial cell) whole cell lysate at 40 µg

Predicted band size: 139 kDa

Observed band size: 140 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20911-169

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "To get stronger band in western blot, we recommend using freshly made lysate to avoid degradation. Use SDS loading buffer to scrape cell from cell culture plate and boil cell lysate 10 minutes immediately. Incubate primary antibody for 3 hours at room temperature room and incubate secondary antibody overnight. Use higher sensitivity ECL substrate and exposure for longer time.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed : 28222186, PubMed : 28877472, PubMed : 30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed : 30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (PubMed : 30111836, PubMed : 35868845, PubMed : 38060563). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed : 27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed : 15883161). May play a role in ciliogenesis (PubMed : 30967659).

See full target information ALPK1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 642:201-211 PubMed40269158

2025

Microbial metabolite drives ageing-related clonal haematopoiesis via ALPK1.

Applications

Unspecified application

Species

Unspecified reactive species

Puneet Agarwal,Avery Sampson,Kathleen Hueneman,Kwangmin Choi,Niels Asger Jakobsen,Emma Uible,Chiharu Ishikawa,Jennifer Yeung,Lyndsey Bolanos,Xueheng Zhao,Kenneth D Setchell,David B Haslam,Jessica Galloway-Pena,John C Byrd,Paresh Vyas,Daniel T Starczynowski

iScience 28:111563 PubMed39868044

2025

IFN-γ licenses normal and pathogenic ALPK1/TIFA pathway in human monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Martin,Solène Caron,Mélissa Marcotte,Pauline Bronnec,Etienne Garneret,Nora Martel,Georgina Maalouf,Pascal Sève,David Saadoun,Yvan Jamilloux,Thomas Henry

Proceedings of the National Academy of Sciences of the United States of America 120:e2313148120 PubMed38060563

2023

ALPK1 mutants causing ROSAH syndrome or Spiradenoma are activated by human nucleotide sugars.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Snelling,Anton Saalfrank,Nicola T Wood,Philip Cohen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALPK1 抗体 [EPR20911-169]

Anti-ALPK1 antibody [EPR20911-169]

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

Western blot - Anti-ALPK1 antibody [EPR20911-169] (AB236626)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise