Anti-alpha Tubulin 抗体 [TU-01] (ab7750)
Key features and details
- Mouse monoclonal [TU-01] to alpha Tubulin
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha Tubulin antibody [TU-01]
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TU-01] to alpha Tubulin -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Turkey, Pig, Saccharomyces cerevisiae, Paramecium tetraurelia, Nicotiana benthamiana -
免疫原
Full length native protein (purified) corresponding to Pig alpha Tubulin aa 37-154.
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エピトープ
aa 65-97 on N-terminal structural domain -
ポジティブ・コントロール
- ICC/IF: HeLa and NIH/3T3 cells. IHC-P: Human skin tissue. Flow Cyt (Intra): HEK293 cells, HeLa cells. WB: Jurkat, HeLa, HEK293T, U87-MG all under reducing conditions.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
TU-01 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7750の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (5) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
reducing conditions. |
特記事項 |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. reducing conditions. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- Unigene: 75318 Human
- Unigene: 1155 Mouse
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別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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Separation of HeLa cells stained using ab7750 (concentration in sample 3 μg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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ICC/IF image of ab7750 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7750, 5µg/ml) overnight at +4°C. The secondary antibody (green) was anti-mouse DyLight® 488 (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with a retrieval buffer EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with an amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blotting analysis labeling alpha-tubulin using ab7750 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of ab74696 followed by IRDye800-conjugated streptavidin. A specific band was detected for alpha-tubulin at approximately 54 kDa.
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Overlay histogram showing HEK293 cells stained with ab7750 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7750, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was anti-mouse DyLight® 488 (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
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Immunocytochemistry/ Immunofluorescence analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. Vimentin was stained red as counterstain. DAPI was used to stain the nuclei blue.
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Immunocytochemistry analysis of HeLa (human cervix carcinoma) cells labelling alpha tubulin (red) with ab7750. DAPI was used to stain the nuclei blue.
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Immunocytochemistry analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. DAPI was used to stain the nuclei blue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab7750 は 32 報の論文で使用されています。
- Tang X et al. The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia-reperfusion injury via regulating NR2B-ERK/CREB signaling. Brain Behav 13:e2867 (2023). PubMed: 36585899
- Robledo E et al. Staphylococcus aureus phagocytosis is affected by senescence. Front Aging 4:1198241 (2023). PubMed: 37584054
- Ramezanpour M et al. Characterization of human nasal organoids from chronic rhinosinusitis patients. Biol Open 11:N/A (2022). PubMed: 35452072
- Yunes SA et al. Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteins. PLoS One 17:e0268857 (2022). PubMed: 35704642
- Huang W et al. Maize cytosolic invertase INVAN6 ensures faithful meiotic progression under heat stress. New Phytol 236:2172-2188 (2022). PubMed: 36104957