AB184970

Anti-alpha Tubulin 抗体 [EPR13799]

Anti-alpha Tubulin antibody [EPR13799] - Loading Control

RabMAb
Recombinant
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(31 Publications)

Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 31 publications.

別名を表示する

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

1 Images

Supplier Data

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (AB184970)

All lanes:

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (ab184970) at 1/50000 dilution

All lanes:

Saccharomyces cerevisiae extract at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13799

アイソタイプ

IgG

キャリアフリー

交差種

Saccharomyces cerevisiae

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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文献 (31)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1782 PubMed39972035

2025

Structural basis of urea transport by Arabidopsis thaliana DUR3.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong An,Yiwei Gao,Laihua Liu,Qinru Bai,Jun Zhao,Yan Zhao,Xuejun C Zhang

Scientific reports 14:11497 PubMed38769106

2024

Upregulation of the AMPK-FOXO1-PDK4 pathway is a primary mechanism of pyruvate dehydrogenase activity reduction in tafazzin-deficient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuqing Liang,Tyler Ralph-Epps,Michael W Schmidtke,Pablo Lazcano,Simone W Denis,Mária Balážová,Nevton Teixeira da Rosa,Mohamed Chakkour,Sanaa Hazime,Mindong Ren,Michael Schlame,Riekelt H Houtkooper,Miriam L Greenberg

Nature microbiology 9:1738-1751 PubMed38649409

2024

The nematode-trapping fungus Arthrobotrys oligospora detects prey pheromones via G protein-coupled receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Yen Kuo,Rebecca J Tay,Hung-Che Lin,Sheng-Chian Juan,Guillermo Vidal-Diez de Ulzurrun,Yu-Chu Chang,Jason Hoki,Frank C Schroeder,Yen-Ping Hsueh

Molecular cell 84:386-400.e11 PubMed38103558

2023

Ubiquiton-An inducible, linkage-specific polyubiquitylation tool.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Renz,Evrydiki Asimaki,Cindy Meister,Véronique Albanèse,Kirill Petriukov,Nils C Krapoth,Sabrina Wegmann,Hans-Peter Wollscheid,Ronald P Wong,Amitkumar Fulzele,Jia-Xuan Chen,Sébastien Léon,Helle D Ulrich

BMC complementary medicine and therapies 23:461 PubMed38102661

2023

Proteomic analysis reveals the molecular mechanism of Astragaloside in the treatment of non-small cell lung cancer by inducing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Liu,Yan Sun,Wenjing Chen,Lingling Deng,Mengmeng Chen,Jingcheng Dong

Molecular cell 83:4461-4478.e13 PubMed38029752

2023

A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Carminati,Juan B Rodríguez-Molina,M Cemre Manav,Dom Bellini,Lori A Passmore

Nature communications 14:4487 PubMed37495621

2023

Architecture and autoinhibitory mechanism of the plasma membrane Na/H antiporter SOS1 in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Wang,Chengcai Pan,Qihao Chen,Qing Xie,Yiwei Gao,Lingli He,Yue Li,Yanli Dong,Xingyu Jiang,Yan Zhao

iScience 26:106635 PubMed37138775

2023

Differential Hsp90-dependent gene expression is strain-specific and common among yeast strains.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Hsiang Hung,Chia-Wei Liao,Fu-Hsuan Ko,Huai-Kuang Tsai,Jun-Yi Leu

EMBO reports 24:e56019 PubMed37009824

2023

Rapid evolutionary repair by secondary perturbation of a primary disrupted transcriptional network.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Chen Hsu,Yu-Hsuan Cheng,Chia-Wei Liao,Richard Ron R Litan,Yu-Ting Jhou,Florica Jean Ganaden Opoc,Ahmed A A Amine,Jun-Yi Leu

PLoS genetics 18:e1010576 PubMed36574460

2022

Stress combined with loss of the Candida albicans SUMO protease Ulp2 triggers selection of aneuploidy via a two-step process.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia Rizzo,Natthapon Soisangwan,Samuel Vega-Estevez,Robert Jordan Price,Chloe Uyl,Elise Iracane,Matt Shaw,Jan Soetaert,Anna Selmecki,Alessia Buscaino

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Tubulin 抗体 [EPR13799]

Anti-alpha Tubulin antibody [EPR13799] - Loading Control

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (AB184970)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (31)

代替製品

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組合せ製品

Primary Antibodies

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Abcam product promise