Anti-alpha Tubulin 抗体 [B-5-1-2] (ab11304)
![Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)](https://www.abcam.co.jp/ps/products/11/ab11304/Images/ab11304-209528-anti-alpha-tubulin-antibody-b-5-1-2-immunofluorescence.jpg "Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)")
Key features and details
- Mouse monoclonal [B-5-1-2] to alpha Tubulin
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-alpha Tubulin antibody [B-5-1-2]
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-5-1-2] to alpha Tubulin -
由来種
Mouse -
アプリケーション
適用あり: ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Pig, Xenopus laevis, Cynomolgus monkey, African green monkey, Xenopus tropicalis
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免疫原
Full length native protein (purified) corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
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エピトープ
The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform. -
ポジティブ･コントロール
- 293T whole cell lysate (ab95494) can be used as a positive control in WB. Total cell extract of the Human foreskin fibroblast cell line (FS11).
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特記事項
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
B-5-1-2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11304の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)
ab11304 staining alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.
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![Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)](https://www.abcam.co.jp/ps/products/11/ab11304/Images/ab11304-18874-anti-alpha-tubulin-antibody-b-5-1-2-immunofluorescence.jpg)
Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)This image is courtesy of an Anonymous Abreviewab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab11304 は 54 報の論文で使用されています。
- Friščić J et al. Reset of Inflammatory Priming of Joint Tissue and Reduction of the Severity of Arthritis Flares by Bromodomain Inhibition. Arthritis Rheumatol 75:517-532 (2023). PubMed: 36245290
- Albrecht M et al. The crosstalk between glomerular endothelial cells and podocytes controls their responses to metabolic stimuli in diabetic nephropathy. Sci Rep 13:17985 (2023). PubMed: 37863933
- Umair Z et al. MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency. Cells 11:N/A (2022). PubMed: 36139370
- Abdul Khaliq S et al. C-Peptide Promotes Cell Migration by Controlling Matrix Metallopeptidase-9 Activity Through Direct Regulation of ß-Catenin in Human Endometrial Stromal Cells. Front Cell Dev Biol 10:800181 (2022). PubMed: 35127683
- Yamamoto-Imoto H et al. Age-associated decline of MondoA drives cellular senescence through impaired autophagy and mitochondrial homeostasis. Cell Rep 38:110444 (2022). PubMed: 35235784