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AB289877

Anti-alpha Tubulin (acetyl K40) 抗体 [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free

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Goat Recombinant Monoclonal Alpha-tubulin 4 acetyl K40 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

別名を表示する

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution.

Positive staining on human cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours (Red) / Untreated control (Green) cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/1000 compared with a Goat IgG / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in HeLa cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling alpha Tubulin acetyl k40 with ab289875 at 1/100 (9.83 μg/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins wos used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in C6 cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) at 1/500 dilution.

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)
  • WB

Lab

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) - BSA and Azide free (AB289877)

This data was developed using ab289875, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 15 seconds; Lanes 3-6 : 5.5 seconds; Lanes 7-8 : 3.25 seconds.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (<a href='/products/primary-antibodies/alpha-tubulin-acetyl-k40-antibody-epr16772-goat-igg-chimeric-ab289875'>ab289875</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma), whole cell lysate at 20 µg

Lane 2:

HeLa treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 3:

Untreated NIH/3T3 (Mouse embyro fibroblast cells), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 5:

Untreated C6 (Rat glial tumor cells), whole cell lysate at 20 µg

Lane 6:

C6 treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 7:

Mouse brain tissue lysate at 20 µg

Lane 8:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Rabbit anti-Goat IgG， (H+LPeroxidase conjugated at 1/5000 dilution

Observed band size: 50 kDa

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関連する標識済み抗体及び組成の異なる製品 (2)

  • Unconjugated

    Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

Key facts

宿主種

Goat

クローン性

Monoclonal

クローン番号

EPR16772

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse, Rat

アプリケーション

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

ab289877 is the carrier-free version of ab289875.

This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab179484). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUBA4A acetyl K40
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組合せ製品

Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB179484

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

  • 5
  • 4
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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