Anti-alpha Tubulin (acetyl K40) 抗体 [EPR16772] - Goat IgG (Chimeric) (ab289875)

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: Lane 1-2: 15 seconds Lane 3-6: 5.5 seconds Lane 7-8:3.25 seconds

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling alpha Tubulin acetyl k40 with AB289875 at 1/100 (9.83 μg/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins wos used. 

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling alpha Tubulin acetyl K40 with AB289875 at 1/500 dilution (1.966μg/ml), followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection kit). Positive staining on mouse cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection kit) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling alpha Tubulin acetyl K40 with ab289875 at 1/500 dilution (1.966 μg/ml), followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection kit). Positive staining on rat cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection kit) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized HeLa cells lebelling alpha Tubulin acetyl K40 with ab289875 at 1/100 (9.83 μg/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells treated with 500 ng/ml Trichostatin A for 4 hours is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells lebelling alpha Tubulin acetyl K40 with ab289875 at 1/100 dilution (9.83 μg/ml), followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells treated with 500 ng/ml Trichostatin A for 4 hours.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) at 1/500 (4 μg/ml) dilution.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells lebelling alpha Tubulin acetyl K40 with AB289875 at 1/100 (9.83 μg/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) antibody at 1/500 dilution (4μg/ml)(Green). Confocal image showing cytoplasmic staining in C6 cells treated with 500 ng/ml Trichostatin A for 4 hours is observed.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) at 1/500 dilution (4 μg/ml).

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours (Red) / Untreated control (Green) cells labelling alpha Tubulin acetyl k40 with AB289875 at 1/1000 dilution (0.1 μg) (Red) and Green (Red) compared with a Goat IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor^® 488, ab150133) at 1/2000 dilution was used as the secondary antibody.