Anti-alpha Tubulin (acetyl K40) 抗体 [EPR16772] - Goat IgG (Chimeric)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution.

Positive staining on human cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours (Red) / Untreated control (Green) cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/1000 compared with a Goat IgG / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in HeLa cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) at 1/500 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling alpha Tubulin acetyl k40 with ab289875 at 1/100 (9.83 μg/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on rat cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling alpha Tubulin (acetyl K40) with ab289875 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on mouse cerebrum.

The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) at 1/500 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labelling alpha Tubulin (acetyl K40) with ab289875 at 1/100 dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) antibody at 1/500 dilution (Green).

Confocal image showing cytoplasmic staining in C6 cells treated with 500 ng/ml Trichostatin A for 4 hours.

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor^® 488) (ab150129) at 1/500 dilution.

• WB

Lab

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (AB289875)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 15 seconds; Lanes 3-6 : 5.5 seconds; Lanes 7-8 : 3.25 seconds.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Goat IgG (Chimeric) (ab289875) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma), whole cell lysate at 20 µg

Lane 2:

HeLa treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 3:

Untreated NIH/3T3 (Mouse embyro fibroblast cells), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 5:

Untreated C6 (Rat glial tumor cells), whole cell lysate at 20 µg

Lane 6:

C6 treated with 500 ng/ml Trichostatin A for 4 hours, whole cell lysate at 20 µg

Lane 7:

Mouse brain tissue lysate at 20 µg

Lane 8:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Rabbit anti-Goat IgG， (H+LPeroxidase conjugated at 1/5000 dilution

Observed band size: 50 kDa

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