Anti-alpha Tubulin (acetyl K40) 抗体 [EPR16772]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells treated with 500 ng/ml Trichostatin A for 4 hourslabeling alpha Tubulin (acetyl K40)with ab179484 at 1/240 dilution (red line). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab179484 preincubated with 1mg/ml acetyl Alpha tubulin (acetyl K40) peptide (green) or non-acetyl Alpha tubulin (acetyl K40) peptide (orange). The isotype control wasRabbit monoclonal IgG (black) and the unlabelled contol was cells without incubation with primary antibody and secondary antibody (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Human brain tissue. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

ab179484 staining alpha Tubulin in HFF-1 (Human skin fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in HFF-1 cells treated with starvation for 48 hours.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

ab179484 stained in Hela cells. Untreated and Trichostatin A treated (50ug/ml, 4 hours) cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab179484 at 1/500 dilution overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43μM for 1hour at room temperature.

• IP

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Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Alpha Tubulin was immunoprecipitated from 1mg of HeLa cells (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours with ab179484 at 1/70 dilution. Western blot was performed from 10 μg of the immunoprecipitate using ab179484 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Hela whole cell extract. Right lane : PBS instead of Hela whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

Predicted band size: 50 kDa

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• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing midbody (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence).The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Mouse cerebral cortex tissue. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on Purkinje cells of cerebellum. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Lane 7:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

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• WB

Supplier Data

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours at 10 µg

Lane 2:

Untreated HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

Lane 1:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours at 10 µg

Lane 2:

Untreated NIH/3T3 whole cell lysate at 10 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Lane 2:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours at 10 µg

Lane 2:

Untreated C6 whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

• WB

CiteAb

Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

alpha Tubulin (acetyl K40) western blot using anti-alpha Tubulin (acetyl K40) antibody [EPR16772] ab179484. Publication image and figure legend from Goldfarb, A. N., Freeman, K. C., et al., 2021, Nat Commun, PubMed 33712594.

ab179484 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab179484 please see the product overview.

Cooperative restoration of FTH1 by isocitrate and fumarate.a FTH1 levels by immunoblot on whole cell lysates from human CD34+ progenitors cultured 3 days in iron-replete (100% TSAT) or deficient (10% TSAT) erythroid medium ± 1–5 mM isocitrate (IC) and ±1 mM fumarate (Fum or F). Colored boxes highlight synergy of 3 mM IC with 1 mM Fum. Red : iron restriction with no or single metabolite treatment; green : iron restriction with combined metabolite treatment. Representative results from three independent experiments. b Graph : mean normalized FTH1 signal (±3 mM IC and ±1 mM Fum), relative to value with 100% TSAT. Error bars, SEM; n = 3 biologically independent experiments; *, **, ***P = 0.02, 0.009, 0.0009, one-way ANOVA with Tukey post hoc. NS not significant. See also Supplementary Fig. 9. Source data are provided as a Source data file.

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