AB317366

Anti-Alpha-synuclein (phospho Y39) 抗体 [EPR25118-5]

Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5]

20ul selling size
Recombinant
RabMAb
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Alpha-synuclein phospho Y39 antibody. Suitable for WB, IP, Dot and reacts with Transfected cell lysate - Mouse, Transfected cell lysate - Human, Synthetic peptide - Human samples.

別名を表示する

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

5 Images

Supplier Data

Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Alpha-synuclein (phospho Y39) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate with ab317366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317366 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lane 2 : ab317366 IP in 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317366 in 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate

All lanes:

Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/30 dilution

All lanes:

293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5s

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with humanTyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein (WT) expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate at 4 µg

Lane 2:

293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein (mutant Y39) protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 4 µg

Lane 2:

293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 8s

Lab

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (25kDa) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 2:

293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a mouse Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Lane 4:

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 6s

Supplier Data

Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Dot blot analysis of Alpha-synuclein (phospho Y39) using ab317366 at 1 : 1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

All lanes:

Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

Lane 1:

Human Alpha-synuclein (phospho Y39) peptide a

Lane 2:

Human Alpha-synuclein (phospho Y39) peptide b

Lanes 3 and 5:

Human Alpha-synuclein non-phospho peptide

Lane 4:

Human Alpha-synuclein (phospho S131) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25118-5

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

WB, IP, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA phospho Y39

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein (phospho Y39) 抗体 [EPR25118-5]

Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5]

Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise