Anti-Alpha-synuclein (phospho S129) 抗体 [P-syn/81A] (ab184674)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674)](https://www.abcam.co.jp/ps/products/184/ab184674/Images/ab184674-2-anti-alpha-synuclein-antibody-phospho-s129-psyn-immunohistochemistry-cerebral-cortex-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674)")
Key features and details
- Mouse monoclonal [P-syn/81A] to Alpha-synuclein (phospho S129)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A]
Alpha-synuclein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [P-syn/81A] to Alpha-synuclein (phospho S129) -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human Alpha-synuclein aa 100 to the C-terminus (phospho S129) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: P37840 -
ポジティブ･コントロール
- IHC-P: Diseased human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
P-syn/81A -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
1/500 - 1/1000. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
組織特異性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
関連疾患
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
配列類似性
Belongs to the synuclein family. -
ドメイン
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻訳後修飾
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
細胞内局在
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 461375 Chimpanzee
- Entrez Gene: 102119369 Cynomolgus monkey
- Entrez Gene: 101146366 Gorilla
- Entrez Gene: 6622 Human
- Entrez Gene: 100190854 Orangutan
- Entrez Gene: 641350 Pig
- Entrez Gene: 29219 Rat
- Entrez Gene: 706985 Rhesus monkey
see all -
別名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674)
Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab184674, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674)](https://www.abcam.co.jp/ps/products/184/ab184674/Images/ab184674-215628-anti-alpha-synuclein-phospho-s129-antibody-p-syn-81a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674)Immunohistochemical analysis of formalin-fixed, paraffin-embedded diseased human brain tissue labeling alpha Synuclein (phospho S129) with ab184674 at 1/500 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab184674 は 31 報の論文で使用されています。
- Gribaudo S et al. Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions. Methods Mol Biol 2551:357-378 (2023). PubMed: 36310215
- Obergasteiger J et al. The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology. NPJ Parkinsons Dis 9:44 (2023). PubMed: 36973269
- Feller B et al. α-Synuclein Preformed Fibrils Bind to β-Neurexins and Impair β-Neurexin-Mediated Presynaptic Organization. Cells 12:N/A (2023). PubMed: 37048156
- Fang TZ et al. Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson's disease mouse model. Nat Commun 14:7295 (2023). PubMed: 37957154
- Won SY et al. p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1. Cell Death Dis 13:575 (2022). PubMed: 35773260