Anti-Alpha-synuclein (phospho S129) 抗体 (ab59264)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho S129)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Alpha-synuclein (phospho S129) antibody
Alpha-synuclein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Alpha-synuclein (phospho S129) -
由来種
Rabbit -
特異性
Detects endogenous levels of Synuclein only when phosphorylated at serine 129. Due to 69% sequence homology ab59264 might react with Beta synuclein.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Alpha-synuclein (phospho S129). Synthetic phosphopeptide derived from Human Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).
Database link: P37840 -
ポジティブ･コントロール
- Human and mouse brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
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| WB | (3) |
1/500 - 1/1000.
Please see WB protocol details in the image legend. |
| 特記事項 |
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IHC-P
1/50 - 1/100. Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
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WB
1/500 - 1/1000. Please see WB protocol details in the image legend. |
ターゲット情報
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機能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
組織特異性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
関連疾患
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
配列類似性
Belongs to the synuclein family. -
ドメイン
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻訳後修飾
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
細胞内局在
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- Unigene: 21374 Human
- Unigene: 17484 Mouse
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別名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
画像
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Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/500 dilution
Lane 1 : Mouse brain whole cell lysates
Lane 2 : Mouse brain whole cell lysates with immunogen phosphopeptide
Lysates/proteins at 40 µg per lane.Blocking buffer: 5% (w/v) BSA in TBST.
Primary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide. -
Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)Image courtesy of Sébastien Brot by Abreview.All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution
Lanes 1-3 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
Lanes 4-6 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
Lane 7 : Whole tissue lysate prepared from KO mouse
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 18,19 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (63)
ab59264 は 63 報の論文で使用されています。
- Gribaudo S et al. Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions. Methods Mol Biol 2551:357-378 (2023). PubMed: 36310215
- Chen B et al. UBL3 Interacts with Alpha-Synuclein in Cells and the Interaction Is Downregulated by the EGFR Pathway Inhibitor Osimertinib. Biomedicines 11:N/A (2023). PubMed: 37371780
- Shin YJ et al. Uric acid regulates α-synuclein transmission in Parkinsonian models. Front Aging Neurosci 15:1117491 (2023). PubMed: 37711993
- McFleder RL et al. Brain-to-gut trafficking of alpha-synuclein by CD11c+ cells in a mouse model of Parkinson's disease. Nat Commun 14:7529 (2023). PubMed: 37981650
- Li ZF et al. A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease. Dis Model Mech 15:N/A (2022). PubMed: 35023548