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リコンビナントRabMAb

Anti-Alpha-synuclein, isoform 98 and 126 抗体 [EPR25117-81] - BSA and Azide free (ab290655)

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Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
  • Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25117-81] to Alpha-synuclein, isoform 98 and 126 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free
    Alpha-synuclein, isoform 98 and 126 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25117-81] to Alpha-synuclein, isoform 98 and 126 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    Alpha-synuclein, isoform 98 and 126.

  • アプリケーション

    適用あり: IHC-P, ICC, WB, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Human

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SNCA12-transfected HEK-293T whole cell lysate, SNCA98-transfected HEK-293T whole cell lysate. IHC: SNCA98-HEK-293T cells, SNCA126-HEK-293T cells. ICC: HEK-293T cells transfected with a human alpha-synuclein isoform SNCA98 and SNCA126 expression vector. Flow Cyt (intra): HEK-293T transfected with a human Alpha-synuclein, isoform 126 or Alpha-synuclein, isoform 98.

  • 特記事項

    ab290655 is a carrier free version of ab290644. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25117-81

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290655の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

  • 組織特異性

    Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

  • 関連疾患

    Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body

  • 配列類似性

    Belongs to the synuclein family.

  • ドメイン

    The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.

  • 翻訳後修飾

    Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

  • 細胞内局在

    Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alpha-synuclein antibody
      • NACP antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A4 component of amyloid precursor antibody
      • SNCA antibody
      • SYUA_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      All lanes : Anti-MAVS antibody [EPR26357-91] (BSA and Azide free) (ab290744) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) cells transfected with a human SNCA140 expression vector containing a myc-His tag whole cell lysate
      Lane 2 : HEK-293T cells transfected with a human SNCA126 expression vector containing a myc-His tag whole cell lysate
      Lane 3 : HEK-293T cells transfected with a human SNCA112 expression vector containing a myc-His tag whole cell lysate
      Lane 4 : HEK-293T cells transfected with a human SNCA98 expression vector containing a myc-His tag whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa


      Exposure time: 3 minutes


      This data was developed using ab290744, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration was 5% NFDM/TBST. 

      This blot was developed using a higher sensitivity ECL substrate. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

      This data was developed using ab290644, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/100 (4.81 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T cells transfected with a SNCA98 expression vector containing a his tag and (B) HEK-293T cells transfected with a SNCA126 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with a SNCA140 expression vector containing a his tag, (D) HEK-293T cells transfected with a SNCA112 expression vector containing a his tag and (E) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab290644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

      This data was developed using ab290644, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA98 and SNCA126 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

      This data was developed using ab290644, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor=® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA140 or SNCA112 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

      This data was developed using ab290644, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 140 (Left) or Alpha-synuclein, isoform 112 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution/ Left and Right compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. No cross-reactivity with Alpha-synuclein, isoform 140 and isoform 112.

    • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

      This data was developed using ab290644, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 126 (Left) or Alpha-synuclein, isoform 98 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution. Left and Right compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)
      Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (ab290655)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290655 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290655 は論文での使用が確認できていません。

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