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Anti-Alpha Skeletal Muscle Actin 抗体 [Alpha Sr-1] (ab28052)

Reviews (5)Q&A (2)References (50)
Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Key features and details

  • Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • Suitable for: WB, ICC
  • Reacts with: Rat, Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
    Alpha Skeletal Muscle Actin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin

  • 由来種

    Mouse

  • 特異性

    In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).

  • アプリケーション

    適用あり: WB, ICCmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Purified striated muscle actin from rabbit.

  • ポジティブ･コントロール

    • WB: Whole extract of Rat heart ICC: HeLa cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab28052の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ICC
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ICC
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • 関連疾患

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.

  • 配列類似性

    Belongs to the actin family.

  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 参照データベース

    • 別名

      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin, alpha skeletal muscle antibody
      • ACTS_HUMAN antibody
      • Alpha actin 1 antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • MPFD antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    画像

    • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
    • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Lane 1 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/500 dilution
      Lane 2 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/1000 dilution
      Lane 3 : Mouse Monoclonal Anti-Actin (a-Sarcomeric) antibody at 1/500 dilution

      All lanes : Whole extract of Rat heart

      Secondary
      All lanes : Goat Anti-Mouse IgG-Peroxidase

      Predicted band size: 42 kDa



      chemiluminescent substrate.

    • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.

      ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

      This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

      See Abreview

    • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
      Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.
      All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

      All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

      Secondary
      All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 1 second


      This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

      See Abreview

    参考文献 (50)

    ab28052 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab28052 は 50 報の論文で使用されています。

    • Chen H  et al. IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary Fibrosis. Aging Dis 14:2215-2237 (2023). PubMed: 37199578
    • O'Brien E  et al. Pharmacological EZH2 inhibition combined with retinoic acid treatment promotes differentiation and apoptosis in rhabdomyosarcoma cells. Clin Epigenetics 15:167 (2023). PubMed: 37858275
    • Huang M  et al. Regulation of Myogenesis by a Na/K-ATPase α1 Caveolin-Binding Motif. Stem Cells 40:133-148 (2022). PubMed: 35257186
    • Wsol A  et al. Increased oxytocinergic system activity in the cardiac muscle in spontaneously hypertensive SHR rats. Arch Med Sci 18:1088-1094 (2022). PubMed: 35832696
    • Zhou J  et al. Sirt1 overexpression improves senescence-associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL-11 transcription. Aging Cell 21:e13680 (2022). PubMed: 35906886
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin [Alpha Sr-1] antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rabbit Tissue sections (Heart)
    Specification
    Heart
    Permeabilization
    No
    Fixative
    Formalin 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Nawazish Naqvi

    Verified customer

    投稿 Jul 14 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Specification
    Heart
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 01 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Branchiostoma belcheri Tissue sections (whole-mount)
    Specification
    whole-mount
    Fixative
    Chilled 100% methanol or 4% paraformaldehyde/MOPS pH 7.5 overnight at 4°C, then stored in 75% Ethanol at -20°C
    Antigen retrieval step
    None
    Permeabilization
    Yes - 5% triton-X 100 / PBS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    XXXXXX XXXXXX

    Verified customer

    投稿 May 10 2010

    Western blot abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Neonatal Cardiac Myocytes)
    Loading amount
    40 µg
    Specification
    Neonatal Cardiac Myocytes
    Treatment
    phenylephrine for 24 hours
    Gel Running Conditions
    Reduced Denaturing (10% polyacrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Richard Patten

    Verified customer

    投稿 Jun 02 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Neonatal Cardiac Myocytes)
    Specification
    Neonatal Cardiac Myocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% NP40
    Blocking step
    2%BSA, 2% FBS in PBS with 0.1% NP40 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Richard Patten

    Verified customer

    投稿 Jun 02 2008

    Question

    Hi We use a lot of your NBR1 antibody listed below. I was wondering if there was a possibility of ordering a larger size? perhaps 100ug or 200ug instead of 50ug? If not, perhaps a quote if we order 3 lots in 1 go? BW  

    Read More
    Answer

    Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I can recommend to contact our customer service department with a completed  list of which antibodies and the quantity required for each product. They will be happy to then provide you with a quote. Please email: orders@abcam.com Thank you for your interest in our products. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    I would like to ask you for help. We need a specific antibody only labeling cardiomyocytes. If possible, the antibody should be labeled already. I have already bought the TroponinI antibody (ab97711) of Abcam which is working quite well. If you could offer another one as desribed, I would be glad. Thank you in advance for your help! Kind regards

    Read More
    Answer

    Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052) - N-Cadherin: N Cadherin antibody (ab12221) - alpha actinin; alpha Actinin antibody [0.T.02] (ab18061) - bFGF: FGF basic antibody (ab16828) I would recommend typing the target name in the search box provided on the Abcam homepage and then selecting the antibodies as per your requirements e.g. monoclonal, polyclonal, rabbit or mouse etc. The full list of Cardiomyocytes markers can be found by clicking the below link; http://www.antibodybeyond.com/reviews/cell-markers/cardiomyocyte-marker.htm Please click on the following link to explore the Abcam Cardiovascular page https://www.abcam.com/index.html?pageconfig=resource&rid=10694 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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