AB189254

Anti-alpha Sarcoglycan 抗体 [EPR14773]

Anti-alpha Sarcoglycan antibody [EPR14773]

RabMAb
Recombinant
20ul selling size
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(16 Publications)

Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) is a rabbit monoclonal antibody detecting alpha Sarcoglycan in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

別名を表示する

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of human skeletal muscle tissue lysate.

All lanes:

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

All lanes:

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

All lanes:

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/10000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14773

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of alpha Sarcoglycan?
Anti-alpha Sarcoglycan [EPR14773] (ab189254) specifically detects a band for alpha Sarcoglycan (UniProt: Q16586) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-alpha Sarcoglycan [EPR14773] (ab189254) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPR14773] also available for your convenience: ab189254, Carrier free - ab240307, PE - ab309435, Alexa Fluor® 647 - ab320777, Alexa Fluor® 488 - ab321851, HRP - ab323780

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.

Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCA

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40183632

2025

AOC 1044 induces exon 44 skipping and restores dystrophin protein in preclinical models of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Usue Etxaniz,Isaac Marks,Tyler Albin,Matthew Diaz,Raghav Bhardwaj,Aaron Anderson,Olecya Tyaglo,Tiffany Hoang,Maria Azzurra Missinato,Kristoffer Svensson,Ben Badillo,Philip R Kovach,Laura Leung,Michael Cochran,Hae Won Kwon,Md Nur Ahad Shah,Rika Maruyama,Toshifumi Yokota,Venkata R Doppalapudi,Beatrice Darimont,Husam S Younis,W Michael Flanagan,Arthur A Levin,Hanhua Huang,Georgios Karamanlidis

Materials today. Bio 28:101180 PubMed39221216

2024

A photothermal responsive system accelerating nitric oxide release to enhance bone repair by promoting osteogenesis and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yannan Cheng,Yuanfang Huo,Yongle Yu,Ping Duan,Xianzhen Dong,Zirui Yu,Qiang Cheng,Honglian Dai,Zhenyu Pan

Experimental and therapeutic medicine 27:97 PubMed38356676

2024

Identification and functional characterization of a novel heterozygous splice‑site mutation in the calpain 3 gene causes rare autosomal dominant limb‑girdle muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Mao,Jie Yang,Xiaodong Zhao,Xueling Jia,Xin Shi,Lihui Zhao,Santasree Banerjee,Lili Zhang,Xiaoling Ma

ACS medicinal chemistry letters 14:1049-1053 PubMed37583821

2023

Synthesis and Evaluation of Bithiazole Derivatives As Potential α-Sarcoglycan Correctors.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Ribaudo,Marcello Carotti,Alberto Ongaro,Erika Oselladore,Martina Scano,Giuseppe Zagotto,Dorianna Sandonà,Alessandra Gianoncelli

Molecular therapy. Methods & clinical development 30:161-180 PubMed37457303

2023

CRISPR-Cas9 correction in the DMD mouse model is accompanied by upregulation of Dp71f protein.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana V Egorova,Anna V Polikarpova,Svetlana G Vassilieva,Marina A Dzhenkova,Irina M Savchenko,Oleg A Velyaev,Anna A Shmidt,Vladislav O Soldatov,Mikhail V Pokrovskii,Alexey V Deykin,Maryana V Bardina

International journal of molecular sciences 24: PubMed37298068

2023

In-Frame Deletion of Dystrophin Exons 8-50 Results in DMD Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana V Egorova,Ivan I Galkin,Oleg A Velyaev,Svetlana G Vassilieva,Irina M Savchenko,Vyacheslav A Loginov,Marina A Dzhenkova,Diana S Korshunova,Olga S Kozlova,Dmitry N Ivankov,Anna V Polikarpova

Molecular therapy. Methods & clinical development 28:284-299 PubMed36816759

2023

Systemic γ-sarcoglycan AAV gene transfer results in dose-dependent correction of muscle deficits in the LGMD 2C/R5 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Eun Seo,Stephen H Baine,Amber N Kempton,Oliver C Rogers,Sarah Lewis,Kaitlin Adegboye,Alex Haile,Danielle A Griffin,Ellyn L Peterson,Eric R Pozsgai,Rachael A Potter,Louise R Rodino-Klapac

Scientific reports 12:848 PubMed35039573

2022

Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

Applications

Unspecified application

Species

Unspecified reactive species

Anna V Starikova,Victoria V Skopenkova,Anna V Polikarpova,Denis A Reshetov,Svetlana G Vassilieva,Oleg A Velyaev,Anna A Shmidt,Irina M Savchenko,Vladislav O Soldatov,Tatiana V Egorova,Maryana V Bardina

Human molecular genetics 31:499-509 PubMed34505136

2021

CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Scano,Alberto Benetollo,Leonardo Nogara,Michela Bondì,Francesco Dalla Barba,Michela Soardi,Sandra Furlan,Eylem Emek Akyurek,Paola Caccin,Marcello Carotti,Roberta Sacchetto,Bert Blaauw,Dorianna Sandonà

Communications biology 4:427 PubMed33782502

2021

MiR-199-3p enhances muscle regeneration and ameliorates aged muscle and muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Fukuoka,Hiromi Fujita,Kosumo Numao,Yasuko Nakamura,Hideo Shimizu,Masayuki Sekiguchi,Hirohiko Hohjoh

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Sarcoglycan 抗体 [EPR14773]

Anti-alpha Sarcoglycan antibody [EPR14773]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

Abcam product promise