Anti-alpha Internexin 抗体 (ab7259)
Key features and details
- Rabbit polyclonal to alpha Internexin
- Suitable for: WB, IHC-FoFr, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-alpha Internexin antibody
alpha Internexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to alpha Internexin -
由来種
Rabbit -
特異性
Specifically recognizes a-internexin. -
アプリケーション
適用あり: WB, IHC-FoFr, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Cow, Human
交差が予測される動物種: Mammals
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免疫原
Recombinant full length protein corresponding to Rat alpha Internexin.
Database link: P23565 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservatives: 0.01% Thimerosal (merthiolate), 0.05% Sodium azide
Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1.5% BSA -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/10000 - 1/20000. Predicted molecular weight: 66 kDa.
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| IHC-FoFr |
1/500 - 1/1000.
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| IHC-P |
1/2000.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/10000 - 1/20000. Predicted molecular weight: 66 kDa. |
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IHC-FoFr
1/500 - 1/1000. |
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IHC-P
1/2000. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. -
組織特異性
Found predominantly in adult CNS. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation. -
翻訳後修飾
O-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 9118 Human
- Entrez Gene: 226180 Mouse
- Entrez Gene: 24503 Rat
- Omim: 605338 Human
- SwissProt: Q16352 Human
- SwissProt: P46660 Mouse
- SwissProt: P23565 Rat
- Unigene: 500916 Human
see all -
別名
- 66 kDa neurofilament protein antibody
- AINX_HUMAN antibody
- Alpha Inx antibody
see all
画像
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Western blot - Anti-alpha Internexin antibody (ab7259)All lanes : Anti-alpha Internexin antibody (ab7259) at 1/10000 dilution
Lane 1 : Protein standard
Lane 2 : Mouse spinal cord lysate
Lane 3 : Rat spinal cord lysate
Lane 4 : Cow spinal cord lysate
Predicted band size: 66 kDa -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha Internexin antibody (ab7259)Immunofluorescence analysis of rat cerebellum section stained for alpha Internexin using ab7259 at a 1/2000 dilution (green) co-strained with chicken polyclonal antibody to GFAP at a 1/5000 dilution (red). DAPI was used to stain nuclear DNA (blue).
Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)Ab7259 shows red, neuronal progenitor cells, plectin (not one of our antibodies) shows fibroblast marker. Photo courtesy of: Dr. Gerry Shaw University of Florida
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody (ab7259)Ab7259 staining Human normal temporal cortex. Staining is localised to intracellualr compartment.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)ICC/IF image of ab7259 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7259, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab7259 は 7 報の論文で使用されています。
- Kullmann JA et al. Oxygen Tension and the VHL-Hif1a Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit. Neuron 106:607-623.e5 (2020). PubMed: 32183943
- Song S et al. Postnatal accumulation of intermediate filaments in the cat and human primary visual cortex. J Comp Neurol 523:2111-26 (2015). PubMed: 25823892
- Nakamura M et al. A truncating SOD1 mutation, p.Gly141X, is associated with clinical and pathologic heterogeneity, including frontotemporal lobar degeneration. Acta Neuropathol 130:145-57 (2015). PubMed: 25917047
- Karpova A et al. Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus. Cell 152:1119-33 (2013). ICC/IF, WB ; Rabbit . PubMed: 23452857
- Balko JM et al. Profiling of residual breast cancers after neoadjuvant chemotherapy identifies DUSP4 deficiency as a mechanism of drug resistance. Nat Med 18:1052-9 (2012). WB . PubMed: 22683778
- Samara A et al. Proteomics reveal rat hippocampal lateral asymmetry. Hippocampus 21:108-19 (2011). WB ; Rat . PubMed: 20020437
- Snowden JS et al. The clinical diagnosis of early-onset dementias: diagnostic accuracy and clinicopathological relationships. Brain 134:2478-92 (2011). PubMed: 21840888