Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-alpha Internexin 抗体 [2E3] (ab7654)

Reviews (3)Q&A (2)References (3)
Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
  • Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
  • Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)

Key features and details

  • Mouse monoclonal [2E3] to alpha Internexin
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-alpha Internexin antibody [2E3]
    alpha Internexin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2E3] to alpha Internexin

  • 由来種

    Mouse

  • 特異性

    specifically recognizes a-internexin.

  • アプリケーション

    適用あり: WB, Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Mammals

  • 免疫原

    Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).

  • エピトープ

    C-terminal non-helical extension of the protein.

  • ポジティブ･コントロール

    • WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7654の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/100. Predicted molecular weight: 66 kDa.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.
特記事項
WB
1/100. Predicted molecular weight: 66 kDa.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.

ターゲット情報

  • 機能

    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

  • 組織特異性

    Found predominantly in adult CNS.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 発生段階

    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.

  • 翻訳後修飾

    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

画像

  • Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse spinal cord lysate
    Lane 5 : Cow spinal cord lysate

    Predicted band size: 66 kDa

  • Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)
    All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)

    Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
    Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
    Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
    Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
    Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

  • Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)
    Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)
    Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献 (3)

ab7654 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7654 は 3 報の論文で使用されています。

  • Rauch U  et al. Expression of intermediate filament proteins and neuronal markers in the human fetal gut. J Histochem Cytochem 54:39-46 (2006). PubMed: 16087706
  • Uchikado H  et al. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005). PubMed: 15883343
  • Ko TL  et al. The expression of alpha-internexin and peripherin in the developing mouse pineal gland. J Biomed Sci 12:777-89 (2005). PubMed: 16132113

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Internexin antibody [2E3]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 100C, 20 min
Sample
Human Tissue sections (cerebral cortex)
Specification
cerebral cortex
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 01 2014

Western blot abreview for Anti-alpha Internexin antibody [2E3]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 06 2012

Immunohistochemistry (Frozen sections) abreview for Anti-alpha Internexin antibody [2E3]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 12 2012

Question

i am getting double band of alpha internexin at expected molecular weight. i have run 7.5% SDS-PAGE of 11x13cm gel size, tranferred to PVDF membrane and did the western blotting as provided literature with the product.in the provided manual only one band has been shown however i have got two close bands each time.what is the reason of this double band? i am attaching a picture of western blot. my sample is mice brain cytoskeletal fraction.

Read More
Answer

Thank you for contacting us about this issue. I am tempted to say you are detecting internexin +/- phosphorylation but the bands appear to be too far apart to fit that explanation. Do you have an image with the molecular weight markers, assuming you included them in the gel?

What are you using for a secondary antibody? If anti-mouse IgG, have you tried staining a blot of these samples with just the secondary antibody, to check the possibility that one of the bands is the heavy chain of endogenous mouse IgG, assuming your samples are reduced and denatured?

Read More

Question

I would like to test the following antibodies in Apteronotus leptorhynchus via IHC-Fr:
ab7654
ab22040
ab7259
ab118982
ab124327
ab40758

Read More
Answer


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit an Abreview for Apteronotus leptorhynchus and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com