AB190467

Anti-alpha-hemolysin 抗体 [8B7] - N-terminal

Name: Anti-alpha-hemolysin 抗体 [8B7] - N-terminal (ab190467) | Abcam
Brand: Abcam Limited
SKU: AB190467
Price: 60000 JPY
Availability: InStock

Anti-alpha-hemolysin antibody [8B7] - N-terminal

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(15 Publications)

Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) is a mouse monoclonal antibody detecting alpha-hemolysin in Western Blot, Neut. Suitable for Staphylococcus aureus.

- Over 10 publications

別名を表示する

hla, hly, Alpha-hemolysin, Alpha-HL, Alpha-toxin

2 Images

Supplier Data

Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

All lanes:

Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) at 1 µg/mL

Lane 1:

alpha-emolysin at 0.1 µg

Lane 2:

Culture supernatant of USA300

Lane 3:

Negative control USA300 delta Hla strain

Predicted band size: 36 kDa

false

Functional Studies (Neut/Block) - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

FuncS (Neut/Block)

Supplier Data

Functional Studies (Neut/Block) - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

Toxin neutralization : Using a rabbit RBC lysis assay, EC₅₀ of ab190467 for neutralization of 0.3 μg/mL of alpha-hemolysin was determined to be 0.676 μg/mL.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8B7

アイソタイプ

IgG3

軽鎖のタイプ

kappa

キャリアフリー

交差種

Staphylococcus aureus

アプリケーション

WB, FuncS (Neut/Block)

applications

免疫原

Synthetic Peptide within Staphylococcus aureus alpha-hemolysin. The exact immunogen used to generate this antibody is proprietary information.

P09616

特異性

ab190467 does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, ab190467 interacts with Protein A in S. aureus culture supernatant via the Fc region.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "FuncSNeutBlock-species-checked": "testedAndGuaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "ab190467 can neutralize the hemolytic activity of the toxin." } } }

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製品の詳細

What is this antibody validated in?
Anti-alpha-hemolysin antibody [8B7] - N-terminal (ab190467) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Neutralising in Staphylococcus aureus samples.

What is the molecular weight of alpha-hemolysin?
Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) specifically detects a band for alpha-hemolysin (UniProt: P09616) at a molecular weight of 36kDa.

Trusted by the scientific community
Anti-alpha-hemolysin [8B7] - N-terminal (ab190467) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-hemolysin also known as alpha-toxin or alpha-haemolysin is a 33.2 kDa pore-forming toxin produced by Staphylococcus aureus. This toxin forms heptameric transmembrane pores with each unit contributing to the overall function of the complex. These pores disrupt target cell membranes leading to hemolysis by osmotic lysis. Alpha-hemolysin is predominantly expressed in S. aureus strains and plays a significant role in bacterial pathogenicity.

Biological function summary

The activity of alpha-hemolysin disrupts plasma membranes of host cells causing leakage of essential ions and metabolites. This action triggers host cell death through destruction of cell integrity. As a monomer alpha-hemolysin assembles into a homo-heptameric structure on the surface of the target membrane creating a channel that allows uncontrolled ion passage. This activity is an important part of the infection process by S. aureus compromising host cell viability and facilitating bacterial invasion.

Pathways

Alpha-hemolysin is integral to pathogenesis pathways notably the Staphylococcus aureus infection pathway. This toxin works in conjunction with other virulence factors to enhance bacterial survival and proliferation. It interacts with other proteins and surface receptors that modulate immune evasion and tissue colonization such as fibronectin-binding proteins influencing disease progression and immune response.

Alpha-hemolysin contributes to conditions like pneumonia and skin abscesses caused by Staphylococcus aureus infections. The toxin's ability to damage host tissues and immune cells helps pathogenic bacteria establish infections. It is often associated with other toxins such as leukocidins which together enhance the bacterium's capacity to persist in hostile environments and evade host defenses.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis (PubMed : 1587866, PubMed : 20624979). Human RBCs bind much less alpha-toxin than do rabbit RBCs (PubMed : 1587866, PubMed : 20624979). Heptamer oligomerization and pore formation is required for lytic activity (PubMed : 1587866, PubMed : 20624979).

See full target information alpha-hemolysin

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:6059 PubMed39972051

2025

Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Mara D Saenz-de-Juano,Giulia Silvestrelli,Samuel Buri,Léa V Zinsli,Mathias Schmelcher,Susanne E Ulbrich

Nature communications 15:9835 PubMed39537625

2024

Post-translational toxin modification by lactate controls Staphylococcus aureus virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Wang,Yanfeng Liu,Guoxiu Xiang,Ying Jian,Ziyu Yang,Tianchi Chen,Xiaowei Ma,Na Zhao,Yingxin Dai,Yan Lv,Hua Wang,Lei He,Bisheng Shi,Qian Liu,Yao Liu,Michael Otto,Min Li

iScience 26:107942 PubMed37790275

2023

Inflammasome-mediated glucose limitation induces antibiotic tolerance in .

Applications

Unspecified application

Species

Unspecified reactive species

Jenna E Beam,Nikki J Wagner,Kuan-Yi Lu,Joshua B Parsons,Vance G Fowler,Sarah E Rowe,Brian P Conlon

Antibiotics (Basel, Switzerland) 12: PubMed37107061

2023

Prevalence of the SigB-Deficient Phenotype among Clinical Isolates Linked to Bovine Mastitis.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Walzl,Helene Marbach,Darya Belikova,Claus Vogl,Monika Ehling-Schulz,Simon Heilbronner,Tom Grunert

Microbiology spectrum 10:e0206322 PubMed35862951

2022

Mechanisms Behind the Indirect Impact of Metabolic Regulators on Virulence Factor Production in Staphylococcus aureus.

Applications

Unspecified application

Species

Unspecified reactive species

Amelia C Stephens,Lance R Thurlow,Anthony R Richardson

Toxins 14: PubMed35878188

2022

Alpha-Toxin in Deep Tracheal Aspirates-Preliminary Evidence for Its Presence in the Lungs of Sepsis Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Ziesemer,Sven-Olaf Kuhn,Anke Hahnenkamp,Manuela Gerber,Elvira Lutjanov,Matthias Gruendling,Jan-Peter Hildebrandt

Evidence-based complementary and alternative medicine : eCAM 2022:4476339 PubMed35586693

2022

Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant via Decreasing Bacterial Toxin Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Sun,Fengjun Sun,Wei Feng,Qian Wang,Fang Liu,Peiyuan Xia,Xuewen Qiu

Journal of thrombosis and haemostasis : JTH 20:1464-1475 PubMed35303391

2022

α-hemolysin of Staphylococcus aureus impairs thrombus formation.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Jahn,Stefan Handtke,Raghavendra Palankar,Thomas P Kohler,Jan Wesche,Martina Wolff,Janina Bayer,Christiane Wolz,Andreas Greinacher,Sven Hammerschmidt

Biology 11: PubMed35336789

2022

Inflammatory Response of Primary Cultured Bovine Mammary Epithelial Cells to Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Mara D Saenz-de-Juano,Giulia Silvestrelli,Andres Weber,Christian Röhrig,Mathias Schmelcher,Susanne E Ulbrich

Frontiers in microbiology 12:789042 PubMed35145494

2022

Secreted Toxins From Strains Isolated From Keratinocyte Skin Cancers Mediate Pro-tumorigenic Inflammatory Responses in the Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Krueger,Julian Zaugg,Sarah Chisholm,Richard Linedale,Nancy Lachner,Siok Min Teoh,Zewen K Tuong,Samuel W Lukowski,Mark Morrison,H Peter Soyer,Philip Hugenholtz,Michelle M Hill,Ian H Frazer

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha-hemolysin 抗体 [8B7] - N-terminal

Anti-alpha-hemolysin antibody [8B7] - N-terminal

Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

Functional Studies (Neut/Block) - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (15)

Abcam product promise