Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-alpha Defensin 5 抗体 [EPR14309(B)] - BSA and Azide free (ab245946)

Submit a review Submit a question
Western blot - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
  • Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14309(B)] to alpha Defensin 5 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free
    alpha Defensin 5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14309(B)] to alpha Defensin 5 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab245946 is the carrier-free version of ab180515.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14309(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245946の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.

  • 組織特異性

    Paneth cells of the small intestine (at protein level).

  • 配列類似性

    Belongs to the alpha-defensin family.

  • 翻訳後修飾

    Glycosylated.

  • 細胞内局在

    Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt

  • 参照データベース

    • 別名

      • alpha 5 antibody
      • DEF5 antibody
      • DEF5_HUMAN antibody
      • Defa antibody
      • Defa29 antibody
      • DEFA5 antibody
      • Defcr29 antibody
      • Defcr5 antibody
      • Defensin 5 antibody
      • Defensin alpha 5 antibody
      • Defensin alpha 5 Paneth cell specific antibody
      • Defensin alpha 5 preproprotein antibody
      • Defensin antibody
      • Enteric defensin antibody
      • HD 5 antibody
      • HD5 antibody
      • HD5(20-94) antibody
      • HD5(63-94) antibody
      • MGC129728 antibody
      • RD 5 antibody
      see all

    画像

    • Western blot - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
      Western blot - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
      Anti-alpha Defensin 5 antibody [EPR14309(B)] (ab180515) at 1/10000 dilution + Human small intestine lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Predicted band size: 10 kDa



      This data was developed using ab180515, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
      This data was developed using ab180515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling alpha Defensin 5 with ab180515 at 1/4000 dilution. Anti-rabbit HRP secondary antibody was used and counter staining with Hematoxylin performed. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
    • Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)
      Anti-alpha Defensin 5 antibody [EPR14309(B)] - BSA and Azide free (ab245946)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245946 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245946 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab245946.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com