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AB327632

Anti-alpha Amylase antibody [HL1681] - BSA and Azide free

Anti-alpha Amylase antibody [HL1681] - BSA and Azide free

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Rabbit Recombinant Monoclonal AMYP antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse, Rat, Human, Zebrafish samples.

別名を表示する

Pancreatic alpha-amylase, PA, AMY2A

6 Images
Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Human pancreas (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

All lanes:

Human pancreas tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts

Lane 2:

DDDDK-tagged AMY2A transfected (+) 293T whole cell extracts

Lane 3:

DDDDK-tagged AMY2B transfected (+) 293T whole cell extracts

Lane 4:

DDDDK-tagged AMY1A transfected (+) 293T whole cell extracts

Lane 5:

DDDDK-tagged AMY1B transfected (+) 293T whole cell extracts

Lane 6:

DDDDK-tagged AMY1C transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/4000 dilution

Lane 1:

Mouse intestine tissue extracts at 50 µg

Lane 2:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted withab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

Lane 1:

Mouse pancreas tissue extracts at 50 µg

Lane 2:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/5000 dilution

All lanes:

Rat pancreas tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)
  • WB

Supplier Data

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (AB327632)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab327632 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-alpha Amylase antibody [HL1681] - BSA and Azide free (ab327632) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL1681

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human, Zebrafish

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information Pancreatic alpha-amylase
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Abcam product promise

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