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RabMAb

Anti-alpha Adducin (phospho S726) 抗体 [EP734Y] (ab40760)

Submit a review Q&A (4)References (3)
Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
  • Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)

Key features and details

  • Rabbit monoclonal [EP734Y] to alpha Adducin (phospho S726)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-alpha Adducin (phospho S726) antibody [EP734Y]
    alpha Adducin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP734Y] to alpha Adducin (phospho S726)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysate. IHC-P: Human lung tissue. ICC/IF: HeLa cells.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40760の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
特記事項
WB
1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

  • 組織特異性

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

  • 配列類似性

    Belongs to the aldolase class II family. Adducin subfamily.

  • ドメイン

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

画像

  • Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    All lanes : Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 120 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 7 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    Western blot - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 120 kDa
    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)

    ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760)
    ab40760, (1/250) staining HeLa cells by immunofluorescence.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab40760 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40760 は 3 報の論文で使用されています。

  • Zhi R  et al. PRMT3 regulates the progression of invasive micropapillary carcinoma of the breast. Cancer Sci 114:1912-1928 (2023). PubMed: 36637351
  • Sheu SH  et al. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell 185:3390-3407.e18 (2022). PubMed: 36055200
  • Thauland TJ  et al. The Actin-Capping Protein Alpha-Adducin Is Required for T-Cell Costimulation. Front Immunol 10:2706 (2019). PubMed: 31824498

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

Thanks for your reply. However, I noticed some contradictions between your discussion and one previous answer in the “Scientific Support” section of ab40760. Posted on 09-November-2006, it said “In regards to ab40760, the immunogenic peptide sequence is proprietary information. However, I can state that it is a 10aa peptide within aa630 - aa700 of the human adducin protein.” I checked the alignment at aa630 - aa700, and found that any 10aa peptide should not result in significant sequence overlap between alpha, beta, and gamma isoforms. Could you please help me find out why this contradiction occurred?

Read More
Answer

Thank you for your reply.

I have had the opportunity to view the exact immunogen sequence used for this product. While the exact information is considered proprietary, I can confirm that this immunogen is based on a region spanning aa 710 through to the C-terminus. I have not found why the post you found from 2006 mentions a different region.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

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Question

Inquiry: Hello Abcam, We purchased three Adducin antibodies from you, and all of them worked very well for our experiments. We are now trying to identify whether they cross-react with different isoforms, and we cannot find this information on your website. So could you please help we clarify (all concerning results on rat, ICC/IF but if you have info on other species/applications, that would be useful, too): 1. For the beta-adducin antibody (ab101668), does it cross-react with alpha- or gamma- adducin? 2. For the two alpha-adducin antibodies we have in hand (ab51130 and ab40760), do they cross-react with beta- or gamma- adducin? Any information would be helpful. Thank you!

Read More
Answer

Sorry for my error. The correct statement is:
The adducin alpha antibody is predicted to cross-react with beta- and gamma-adducin. The immunogen shares 100% identity with both beta- and gamma-adducin, respectively.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

Inquiry: We recently purchased two alpha-adducin antibodies from you (ab51130 and ab40760). We now urgently need to know whether they cross-react with beta- or gamma- adducin. Could you please help us with this question (all concerning results on rat, ICC/IF but if you have info on other species/applications, that would be useful, too)?

Read More
Answer

Thank you for contacting us.

I have collected some data for you regarding these products as requested.
Regarding ab40760; this is predicted to cross-react with Beta-Adducin and Gamma-Adducin, based on sequence analysis. The immunogen shares 100% and 94% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.

Regarding ab51130; this product is also predicted to cross-react with the rat Beta-Adducin and rat Gamma-Adducin, also based on sequence analysis. The immunogen shares 96% and 91% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.

These products are created from immunogens made of the C terminal region of alpha-adducin which shares high homology with the beta- and gamma- forms in mouse, human and rat. As such, we do guarantee them to work in those species in the applications listed on the specific datasheets. We try to include as much information as we have into these datasheets and do not know if these have been tested in other species or applications not listed.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

Read More

Question


Inquiry: Hello Abcam, We purchased three Adducin antibodies from you, and all of them worked very well for our experiments. We are now trying to identify whether they cross-react with different isoforms, and we cannot find this information on your website.

Read More
Answer

The beta-adducin antibody is likely (although not guaranteed) to cross-react with alpha- or gamma- adducin. The immunogen shares 100% and 94% identity with alpha- and gamma-adducin, respectively.
I hope this is helpful. Please contact me again if you have any further questions.

Read More

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