Anti-alpha Adducin (phospho S59) 抗体 [EP2175AY] (ab76251)
![Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)](https://www.abcam.co.jp/ps/products/76/ab76251/Images/ab76251-91620-anti-alpha-adducin-phospho-s59-antibody-ep2175ay-western-blot.jpg "Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha Adducin (phospho S59) antibody [EP2175AY]
alpha Adducin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa cell lysates, untreated, or treated with calyculin A
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2175AY -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76251の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/5000. Predicted molecular weight: 81 kDa. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
ターゲット情報
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機能
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
組織特異性
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
配列類似性
Belongs to the aldolase class II family. Adducin subfamily. -
ドメイン
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
細胞内局在
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 118 Human
- Omim: 102680 Human
- SwissProt: P35611 Human
- Unigene: 183706 Human
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別名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
画像
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Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted? -
![Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)](https://www.abcam.co.jp/ps/products/76/ab76251/Images/ab76251-2-benefits-of-recombinant-antibodies.png)
Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab76251 は 1 報の論文で使用されています。
- Bouyer G et al. Plasmodiumfalciparum infection induces dynamic changes in the erythrocyte phospho-proteome. Blood Cells Mol Dis 58:35-44 (2016). PubMed: 27067487