AB76251

Anti-alpha Adducin (phospho S59) 抗体 [EP2175AY]

Anti-alpha Adducin (phospho S59) antibody [EP2175AY]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal alpha Adducin phospho S59 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha

1 Images

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Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (AB76251)

All lanes:

Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution

Lane 1:

HeLa cell lysate (untreated) at 10 µg

Lane 2:

HeLa cell lysate, treated with calyculin A at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP labelled IgG at 1/1000 dilution

Predicted band size: 81 kDa

Observed band size: 115 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2175AY

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.

Biological function summary

This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.

Pathways

Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.

Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

See full target information ADD1 pS59

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 11:e15549 PubMed36597221

2023

β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Keyvan Karimi Galougahi,Yunjia Zhang,Vivian Kienzle,Chia-Chi Liu,Lake-Ee Quek,Sanjay Patel,Edmund Lau,Rachael L Cordina,Gemma A Figtree,David S Celermajer

Blood cells, molecules & diseases 58:35-44 PubMed27067487

2016

Plasmodiumfalciparum infection induces dynamic changes in the erythrocyte phospho-proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Bouyer,Luc Reininger,Ghania Ramdani,Lee D Phillips,Vikram Sharma,Stephane Egee,Gordon Langsley,Edwin Lasonder

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Adducin (phospho S59) 抗体 [EP2175AY]

Anti-alpha Adducin (phospho S59) antibody [EP2175AY]

Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (AB76251)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise