AB125081

Anti-alpha Adducin (phospho S436) 抗体 [EPR2176]

Anti-alpha Adducin (phospho S436) antibody [EPR2176]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal alpha Adducin phospho S436 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

ADDA, ADD1, Alpha-adducin, Erythrocyte adducin subunit alpha

2 Images

Lab

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (AB125081)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (ab125081) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 110 kDa

false

Exposure time: 7s

Unknown

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (AB125081)

The bands shown at the bottom of the gel are Beta Tubulin.

All lanes:

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (ab125081) at 1/1000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 81 kDa,86 kDa

Observed band size: 90 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2176

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Adducin also known as ADD1 is a cytoskeletal protein of roughly 80 kDa. It plays a significant mechanical role in the assembly of the spectrin-actin network which stabilizes the membrane of erythrocytes and other cells. It is expressed in various tissues with high levels in neurons and kidney cells. Maintenance of cytoskeletal integrity is an important mechanical function of alpha Adducin.

Biological function summary

This protein influences cellular shape and motility. It forms a complex with spectrin and actin to strengthen cell membranes and facilitate intercellular connectivity. Alpha Adducin modulates the dynamics of membrane skeleton structures impacting not only the stability but also the function of cells under both physiological and pathophysiological conditions.

Pathways

Alpha Adducin is integral in the regulation of the Rho and calcium signaling pathways. These pathways influence cytoskeletal organization and cell life processes. In the Rho pathway alpha Adducin interacts with proteins such as Rho GTPases which are involved in signal transduction for cytoskeletal rearrangement. This interaction shows its importance in cellular reaction to environmental stimuli and stress.

Alpha Adducin shows significant connections to hypertension and certain neurological disorders. Variants of ADD1 have been linked to altered blood pressure regulation implicating its role in hypertension through interactions with sodium-potassium ATPases. It also connects to spectrin in neurological disorders highlighting its involvement in maintaining neuronal architecture and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

See full target information ADD1 pS436

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0197266 PubMed29768513

2018

Increased mitochondrial fission is critical for hypoxia-induced pancreatic beta cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhang,Yanfang Liu,Yao Tang,Xiaofeng Wang,Zhichao Li,Rui Li,Zhenyu Ti,Weidong Gao,Jigang Bai,Yi Lv

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Adducin (phospho S436) 抗体 [EPR2176]

Anti-alpha Adducin (phospho S436) antibody [EPR2176]

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (AB125081)

Western blot - Anti-alpha Adducin (phospho S436) antibody [EPR2176] (AB125081)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise