Anti-alpha Actinin/ACTN1 抗体 [EP2528Y] - BSA and Azide free (ab185204)
![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-415170-anti-alpha-actininactn1-antibody-ep2528y-bsa-and-azide-free-western-blot-hap1-wt-empty-hap1-actn1-knockout-a431-daudi.jpg "Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2528Y] to alpha Actinin/ACTN1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free
alpha Actinin/ACTN1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2528Y] to alpha Actinin/ACTN1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HAP1, A431, NIH 3T3, C2C12, C6 and A673 cell lysates and human skeletal muscle and human heart tissue lysates.
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特記事項
ab185204 is the carrier-free version of ab81265.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2528Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 87 Human
- Entrez Gene: 109711 Mouse
- Entrez Gene: 81634 Rat
- Omim: 102575 Human
- SwissProt: P12814 Human
- SwissProt: Q7TPR4 Mouse
- SwissProt: Q9Z1P2 Rat
- Unigene: 509765 Human
see all -
別名
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
see all
画像
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Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ACTN1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab81265).
Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 115 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab81265 was shown to react with ACTN1 in wild-type HAP1 cells in western blot with loss of signal observed in ACTN1 knockout sample. Wild-type and ACTN1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab81265 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-410646-anti-alpha-actinin-actn1-antibody-ep2528y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)This data was developed using ab81265, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Alpha Actinin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: NIH 3T3 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 103 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab81265 was shown to recognize alpha Actinin in wild-type HAP1 cells as signal was lost at the expected MW in alpha Actinin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and alpha Actinin knockout samples were subjected to SDS-PAGE. ab81265 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-407957-anti-alpha-actinin-actn1-antibody-ep2528y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lane 3 : C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaThis data was developed using ab81265, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-405627-anti-alpha-actinin-actn1-antibody-ep2528y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaThis data was developed using ab81265, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-402426-anti-alpha-actinin-actn1-antibody-ep2528y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (unpurified)
Lane 1 : NIH 3T3 cell lysate
Lane 2 : human skeletal muscle tissue lysate
Lane 3 : human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaThis data was developed using ab81265, the same antibody clone in a different buffer formulation.
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![Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)](https://www.abcam.co.jp/ps/products/185/ab185204/Images/ab185204-6-benefits-of-recombinant-antibodies.png)
Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab185204 は論文での使用が確認できていません。
