Anti-alpha Actinin/ACTN1 抗体 [EP2528Y] (ab81265)
![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-415036-anti-alpha-actininactn1-antibody-ep2528y-western-blot-hap1-wt-empty-hap1-actn1-knockout-a431-daudi.jpg "Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2528Y] to alpha Actinin/ACTN1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha Actinin/ACTN1 antibody [EP2528Y]
alpha Actinin/ACTN1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2528Y] to alpha Actinin/ACTN1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human alpha Actinin/ACTN1 aa 750-850 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: HAP1, A431, NIH 3T3, C2C12, C6 and A673 cell lysates and human skeletal muscle and human heart tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2528Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 87 Human
- Entrez Gene: 109711 Mouse
- Entrez Gene: 81634 Rat
- Omim: 102575 Human
- SwissProt: P12814 Human
- SwissProt: Q7TPR4 Mouse
- SwissProt: Q9Z1P2 Rat
- Unigene: 509765 Human
see all -
別名
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
see all
画像
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Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ACTN1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 115 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab81265 was shown to react with ACTN1 in wild-type HAP1 cells in western blot with loss of signal observed in ACTN1 knockout sample. Wild-type and ACTN1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab81265 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-299870-anti-alpha-actinin-antibody-ep2528y-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Alpha Actinin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: NIH 3T3 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 103 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab81265 was shown to recognize alpha Actinin in wild-type HAP1 cells as signal was lost at the expected MW in alpha Actinin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and alpha Actinin knockout samples were subjected to SDS-PAGE. ab81265 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-257344-anti-alpha-actinin-antibody-ep2528y-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lane 3 : C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-257345-anti-alpha-actinin-antibody-ep2528y-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-96445-anti-alpha-actinin-antibody-ep2528y-western-blot.jpg)
Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)All lanes : Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265) at 1/1000 dilution (unpurified)
Lane 1 : NIH 3T3 cell lysate
Lane 2 : human skeletal muscle tissue lysate
Lane 3 : human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
![Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)](https://www.abcam.co.jp/ps/products/81/ab81265/Images/ab81265-7-benefits-of-recombinant-antibodies.png)
Anti-alpha Actinin/ACTN1 antibody [EP2528Y] (ab81265)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab81265 は論文での使用が確認できていません。