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Anti-alpha 2a Adrenergic Receptor 抗体 (ab85570)

Submit a review Q&A (3)References (6)
Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)

Key features and details

  • Rabbit polyclonal to alpha 2a Adrenergic Receptor
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-alpha 2a Adrenergic Receptor antibody

  • 製品の詳細

    Rabbit polyclonal to alpha 2a Adrenergic Receptor

  • 由来種

    Rabbit

  • 特異性

    From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig

  • 免疫原

    Synthetic peptide corresponding to Human alpha 2a Adrenergic Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102061)

  • ポジティブ･コントロール

    • Recombinant human alpha 2a Adrenergic Receptor protein (ab54291) can be used as a positive control in WB. This antibody gave a positive signal in Human pancreas tissue lysates.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98.98% PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab85570の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).
特記事項
IHC-P
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ADA2A_HUMAN antibody
    • ADRA2 antibody
    • ADRA2A antibody
    • ADRA2R antibody
    • ADRAR antibody
    • Adrenergic alpha 2A receptor antibody
    • Alpha 2 adrenergic receptor subtype C10 antibody
    • Alpha 2A adrenergic receptor antibody
    • Alpha 2AAR subtype C10 antibody
    • Alpha-2 adrenergic receptor subtype C10 antibody
    • alpha-2-adrenergic receptor, platelet type antibody
    • Alpha-2A adrenergic receptor antibody
    • Alpha-2A adrenoceptor antibody
    • Alpha-2A adrenoreceptor antibody
    • Alpha-2AAR antibody
    • ALPHA2AAR antibody
    • OTTHUMP00000058849 antibody
    • ZNF32 antibody
    see all

画像

  • Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Anti-alpha 2a Adrenergic Receptor antibody (ab85570) at 2 µg/ml + Human Pancreas at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Blocking buffer: 2% BSA

    Gel type: MOPS

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    IHC image of alpha 2a Adrenergic Receptor staining in human Pancreas FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85570, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

参考文献 (6)

ab85570 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab85570 は 6 報の論文で使用されています。

  • Hu Y  et al. Intrathecal Injection of Ropivacaine Reduces Cervical Resistance in Late-Pregnant Rats. Drug Des Devel Ther 16:1183-1189 (2022). PubMed: 35502424
  • Liu X  et al. Distribution and relative expression of vasoactive receptors on arteries. Sci Rep 10:15383 (2020). PubMed: 32958803
  • Wang W  et al. a2A-Adrenergic Receptor Inhibits the Progression of Cervical Cancer Through Blocking PI3K/AKT/mTOR Pathway. Onco Targets Ther 13:10535-10546 (2020). PubMed: 33116632
  • Yu Z  et al. Single-Cell Transcriptomic Map of the Human and Mouse Bladders. J Am Soc Nephrol 30:2159-2176 (2019). PubMed: 31462402
  • Weng X  et al. Reduced mitochondrial response sensitivity is involved in the anti-apoptotic effect of dexmedetomidine pretreatment in cardiomyocytes. Int J Mol Med 41:2328-2338 (2018). PubMed: 29328437
  • Reed DE  et al. Stress increases descending inhibition in mouse and human colon. Neurogastroenterol Motil 28:569-80 (2016). PubMed: 26744175

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

Could you please make the blocking peptides available for ab8557 and ab45871?

Read More
Answer

The blocking peptide for ab85570 is ab102061.

For ab45871, ab54291 can be used.

Read More

Question

Could you check on the alignment of my protein and sequence of the peptide against which this antibody was raised?

Read More
Answer

BLAST alignment of the immunogen sequence for ab65833, anti-alpha 2a Adrenergic Receptor, with your protein of interest returned a result of 81% identity (13/16).



Please let us know if you have any questions.

Read More

Question

IHC-P with human pancreas: good
with mouse and rat pancreas: faint specific staiing and high background
AR: pH6, pH9 (best), proteinase, none
Ab: 1/400 RT (best) and o/n (too much background)
block: serum-free, 0.25% casein
2nd: OK
block with 3% H2O2

Read More
Answer

Thank you for your phone call.
Here is the information for the discount code for trying ab85570 in mouse and rat. Below that section, please see the information for the free of charge replacement.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85570 in mouse/rat. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

===== FREE of CHARGE REPLACEMENT =====

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab85570.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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