Anti-alpha 1 Spectrin 抗体 [17C7] (ab11751)
Key features and details
- Mouse monoclonal [17C7] to alpha 1 Spectrin
- Suitable for: ELISA, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-alpha 1 Spectrin antibody [17C7]
alpha 1 Spectrin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [17C7] to alpha 1 Spectrin -
由来種
Mouse -
特異性
ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes. -
アプリケーション
適用あり: ELISA, IHC-Fr, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
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ポジティブ・コントロール
- Erythroid spectrin in red blood cells and cerebellum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
17C7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview. |
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa. |
ターゲット情報
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機能
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. -
関連疾患
Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. -
配列類似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. - Information by UniProt
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参照データベース
- Entrez Gene: 6708 Human
- Entrez Gene: 20739 Mouse
- Entrez Gene: 289257 Rat
- Omim: 182860 Human
- SwissProt: P02549 Human
- SwissProt: P08032 Mouse
- Unigene: 119825 Human
- Unigene: 200611 Mouse
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別名
- Alpha I spectrin antibody
- EL 2 antibody
- EL2 antibody
see all
画像
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All lanes : Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/ml
Lane 1 : MW marker
Lane 2 : Human Lung lysate
Lane 3 : Mouse Lung lysate
Lane 4 : Rat Brain lysate
Lane 5 : Human Kidney lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP labeled goat anti-mouse IgG
Predicted band size: 280 kDa -
Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)This image is courtesy of an Abreview submitted by Dr. Mahesh ShivananjappaAnti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg
Secondary
HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 280 kDa
Additional bands at: 200 kDa (possible cleavage fragment)
Exposure time: 1 hour
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (14)
ab11751 は 14 報の論文で使用されています。
- Chen Y et al. Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high-fat diet via Hippo signaling pathway. Andrology 11:591-610 (2023). PubMed: 36374586
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
- Lin YS et al. STIM1 Controls the Focal Adhesion Dynamics and Cell Migration by Regulating SOCE in Osteosarcoma. Int J Mol Sci 23:N/A (2021). PubMed: 35008585
- Smith AS et al. MYH9-related disease mutations cause abnormal red blood cell morphology through increased myosin-actin binding at the membrane. Am J Hematol 94:667-677 (2019). PubMed: 30916803
- Wang Y et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018). PubMed: 29337302