Anti-alpha 1 Sodium Potassium ATPase 抗体 [464.6] (ab7671)
Key features and details
- Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Rabbit, Human, Pig
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
alpha 1 Sodium Potassium ATPase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Human, Pig
交差が予測される動物種: Sheep, Dog, Xenopus laevis, Monkey -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
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特記事項
This antibody clone [464.6] is manufactured by Abcam.
If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
464.6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
- HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696)
- Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (10) |
Use a concentration of 1 - 10 µg/ml.
We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
IHC-P | (5) |
Use a concentration of 5 µg/ml.
|
WB | (23) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa.
Abcam recommends using 5% BSA as the blocking agent. |
特記事項 |
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ICC/IF
Use a concentration of 1 - 10 µg/ml. We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent. |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻訳後修飾
Phosphorylation on Tyr-10 modulates pumping activity. -
細胞内局在
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
別名
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
画像
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Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Armen PetrosyanImmunocytochemistry analysis of formaldehyde-fixed mouse hepatocytes permeabilized with 0.2% Triton X-100 in PBS staining with ab7671 at 1/50 dilution. Secondary antibody was Alexa Fluor™ 488 Donkey anti-Ms. Samples were incubated with the primary antibody with 1% donkey serum in PBST for 3 hours at 22°C. Blocking was done using 1% donkey serum in PBST for 1 hour at 22°C.
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailer
ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate
Predicted band size: 112 kDa
Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (357)
ab7671 は 357 報の論文で使用されています。
- Ruturaj et al. Regulation of the apico-basolateral trafficking polarity of the homologous copper-ATPases ATP7A and ATP7B. J Cell Sci 137:N/A (2024). PubMed: 38032054
- Sun A et al. Molecular Mechanisms of Organic Anion Transporting Polypeptide-Mediated Organic Anion Clearance at the Blood-Cerebrospinal Fluid Barrier. Mol Pharmacol 104:255-265 (2023). PubMed: 37652713
- Gurler B et al. Hypoxia and HIF-1α Regulate the Activity and Expression of Na,K-ATPase Subunits in H9c2 Cardiomyoblasts. Curr Issues Mol Biol 45:8277-8288 (2023). PubMed: 37886965
- Hai Y et al. Icariin Alleviates Nonalcoholic Fatty Liver Disease in Polycystic Ovary Syndrome by Improving Liver Fatty Acid Oxidation and Inhibiting Lipid Accumulation. Molecules 28:N/A (2023). PubMed: 36677577
- Nalbach K et al. Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2. Nat Commun 14:870 (2023). PubMed: 36797266