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リコンビナントRabMAb

Anti-alpha 1 Fetoprotein 抗体 [EPR26548-19] - BSA and Azide free (ab290648)

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Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
  • Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26548-19] to alpha 1 Fetoprotein - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free
    alpha 1 Fetoprotein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26548-19] to alpha 1 Fetoprotein - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Untreated Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate, Hepa1-6 treated with 300ng/ml Brefeldin A for 24h whole cell lysate, Mouse E14 embryo tissue lysate, Mouse placenta tissue lysate, Rat placenta tissue lysate IHC-P: mouse liver E16.5 tissue, mouse placenta tissue, rat placenta tissue.

  • 特記事項

    ab290648 is a carrier free version of ab290637. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290648の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

ターゲット情報

  • 機能

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

  • 組織特異性

    Plasma. Synthesized by the fetal liver and yolk sac.

  • 配列類似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 発生段階

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

  • 翻訳後修飾

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Afp antibody
      • AFPD antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    画像

    • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      All lanes : Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637) at 1/1000 dilution

      Lane 1 : Untreated Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
      Lane 2 : Hepa1-6 treated with 300ng/ml Brefeldin A for 24h whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 68 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      This data was developed using ab290637, the same antibody clone in different buffer formulation. 

      Blocking and dilution buffer and concentration was 5% NFDM/TBST. 



    • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      All lanes : Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (ab290637) at 1/1000 dilution

      Lane 1 : Mouse E14 embryo tissue lysate
      Lane 2 : Mouse placenta tissue lysate
      Lane 3 : Mouse liver tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : Rat placenta tissue lysate
      Lane 6 : Rat liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 68 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 8 seconds


      This data was developed using ab290637, the same antibody clone in different buffer formulation. 

      Blocking and dilution buffer and concentration was 5% NFDM/TBST. 

      Negative control: liver, brain(PMID: 16708394, PMID: 9891062).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

      This data was developed using ab290637, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver E16.5 tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse fetal liver (E16.5) (PMID: 15005571). The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

      This data was developed using ab290637, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse placenta tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining was found on mouse placenta. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

      This data was developed using ab290637, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat placenta tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining was found on rat placenta. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

      This data was developed using ab290637, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on adult mouse liver. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

      This data was developed using ab290637, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labelling alpha 1 Fetoprotein with ab290637 at 1/1000 (0.534 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on adult rat liver. The section was incubated with ab290637 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)
      Anti-alpha 1 Fetoprotein antibody [EPR26548-19] - BSA and Azide free (ab290648)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290648 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290648 は論文での使用が確認できていません。

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