Anti-alpha 1 Fetoprotein 抗体 [EP1017Y] (ab45147)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)](https://www.abcam.co.jp/ps/products/45/ab45147/Images/ab45147-57311-anti-alpha-1-fetoprotein-antibody-ep1017y-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1017Y] to alpha 1 Fetoprotein
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-alpha 1 Fetoprotein antibody [EP1017Y]
alpha 1 Fetoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1017Y] to alpha 1 Fetoprotein -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human alpha 1 Fetoprotein (C terminal). The exact sequence is proprietary.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1017Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 69 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 69 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
-
別名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)
Ab45147 staining human alpha 1 Fetoprotein in human fetal liver by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)](https://www.abcam.co.jp/ps/products/45/ab45147/Images/ab45147-57312-anti-alpha-1-fetoprotein-antibody-ep1017y-western-blot.jpg)
Western blot - Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147) at 1/50000 dilution + HepG2 membrane
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)](https://www.abcam.co.jp/ps/products/45/ab45147/Images/ab45147-3-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab45147 は 1 報の論文で使用されています。
- Joshi M et al. Chemokine-mediated robust augmentation of liver engraftment: a novel approach. Stem Cells Transl Med 4:21-30 (2015). PubMed: 25473087