AB251587

Anti-alpha 1 Fetoprotein 抗体 [CPT-R19-48-7] - BSA and Azide free

Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal alpha 1 Fetoprotein antibody. Carrier free. Suitable for ELISA and reacts with Synthetic peptide, Human samples.

別名を表示する

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

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ELISA - Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] - BSA and Azide free (AB251587)

ELISA

Supplier Data

ELISA - Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] - BSA and Azide free (AB251587)

This data was developed using ab241324, the same antibody clone in a different buffer formulation.

100 ng CPTAC-23a (2000 ng/mL, 50 μL per well) was coated onto 96-wells.

Serial dilutions (0, 0.01, 0.02, 0.05, 0.15, 0.44, 1.33, 4.00 μg/mL) of ab241324 (50 μL) were incubated with CPTAC-23a in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development.

OD was read at 405nm within 5 minutes.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

CPT-R19-48-7

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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製品の詳細

ab251587 is the carrier-free version of ab241324.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein 抗体 [CPT-R19-48-7] - BSA and Azide free

Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] - BSA and Azide free

ELISA - Anti-alpha 1 Fetoprotein antibody [CPT-R19-48-7] - BSA and Azide free (AB251587)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

代替製品

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise