Anti-alpha 1 Fetoprotein 抗体 [AFP-01] (ab3980)
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)](https://www.abcam.co.jp/ps/products/3/ab3980/Images/ab3980-8451-anti-alpha-1-fetoprotein-antibody-afp-01-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)")
Key features and details
- Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-alpha 1 Fetoprotein antibody [AFP-01]
alpha 1 Fetoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein -
由来種
Mouse -
特異性
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
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ポジティブ･コントロール
- ICC/IF: MCF7 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified by precipitation and chromatography -
ポリ/モノ
モノクローナル -
クローン名
AFP-01 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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別名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab3980 は 45 報の論文で使用されています。
- Lee HS et al. Integrative analysis of multiple genomic data from intrahepatic cholangiocarcinoma organoids enables tumor subtyping. Nat Commun 14:237 (2023). PubMed: 36646721
- Kim JH et al. Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids. iScience 26:107675 (2023). PubMed: 37680467
- Cherkashova E et al. Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke. PeerJ 11:e16358 (2023). PubMed: 38025691
- Mao X et al. Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1. Oncol Lett 23:84 (2022). PubMed: 35126726
- Jeong J et al. Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids. Biology (Basel) 11:N/A (2022). PubMed: 35453693