Anti-alpha 1 Antitrypsin 抗体 [G11] (ab9400)
![Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)](https://www.abcam.co.jp/ps/products/9/ab9400/Images/ab9400-17021-anti-alpha-1-antitrypsin-antibody-g11-western-blot.jpg "Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)")
Key features and details
- Mouse monoclonal [G11] to alpha 1 Antitrypsin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-alpha 1 Antitrypsin antibody [G11]
alpha 1 Antitrypsin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [G11] to alpha 1 Antitrypsin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human alpha 1 Antitrypsin.
Database link: P01009 -
特記事項
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR3176514-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
G11 -
ミエローマ
NS0/1 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. |
ターゲット情報
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機能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
組織特異性
Ubiquitous. Expressed in leukocytes and plasma. -
関連疾患
Alpha-1-antitrypsin deficiency -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻訳後修飾
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
細胞内局在
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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別名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
画像
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Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml
Secondary
HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)](https://www.abcam.co.jp/ps/products/9/ab9400/Images/ab9400_1.gif)
Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400)Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab9400 は 14 報の論文で使用されています。
- Patil K et al. Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome. PLoS One 14:e0214742 (2019). PubMed: 30946770
- Cagnone M et al. A Pilot Study to Investigate the Balance between Proteases and a1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients. High Throughput 8:N/A (2019). PubMed: 30781848
- Esteghamat F et al. CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nat Genet 51:1233-1243 (2019). PubMed: 31358993
- Henneuse A et al. a1 -antitrypsin Pittsburgh and plasmin-mediated proteolysis. J Thromb Haemost 14:2023-2026 (2016). PubMed: 27468101
- Kang H et al. Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP. Biochem Biophys Res Commun 460:596-602 (2015). PubMed: 25800869