Anti-ALP 抗体 (ab67228)
Key features and details
- Mouse polyclonal to ALP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALP antibody
ALP 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to ALP -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human ALP. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- ALP transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67228の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
May play a role in the organization of actin filament arrays within muscle cells. -
組織特異性
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
細胞内局在
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines. - Information by UniProt
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参照データベース
- Entrez Gene: 27295 Human
- Omim: 605889 Human
- SwissProt: Q53GG5 Human
- Unigene: 442702 Human
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別名
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
画像
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Western blot - Anti-ALP antibody (ab67228)All lanes : Anti-ALP antibody (ab67228) at 1/500 dilution
Lane 1 : ALP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (20)
ab67228 は 20 報の論文で使用されています。
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Zhang Y et al. miR-126 mitigates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting the ERK1/2 and Bcl-2 pathways. Acta Biochim Biophys Sin (Shanghai) 55:449-459 (2023). PubMed: 36942990
- Li Y et al. LincRNA-EPS increases TGF-β expression to inhibit the Wnt/β-catenin pathway, VSMC osteoblastic differentiation and vascular calcification in diabetic mice. Exp Ther Med 23:425 (2022). PubMed: 35607373
- Li H et al. Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway. Mol Metab 66:101617 (2022). PubMed: 36270612
- Nirwana I et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res 12:116-120 (2022). PubMed: 34840942
