Anti-ALKBH8 抗体 (ab113512)
Key features and details
- Rabbit polyclonal to ALKBH8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ALKBH8 antibody -
製品の詳細
Rabbit polyclonal to ALKBH8 -
由来種
Rabbit -
特異性
ab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa). -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Recombinant fragment, corresponding to N terminal amino acids 2-238 of Human ALKBH8 (BC015183).
-
ポジティブ･コントロール
- Human fetal spleen and A549 cell lysates; Human fetal spleen tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab113512 is purified by a protein affinity column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113512の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
|
|
| IHC-P |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa. |
|
IHC-P
1/100 - 1/500. |
ターゲット情報
-
機能
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. -
組織特異性
Widely expressed, with highest expression in spleen, followed by pancreas and lung. -
配列類似性
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 RRM (RNA recognition motif) domain. -
細胞内局在
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
-
参照データベース
- Entrez Gene: 91801 Human
- Entrez Gene: 67667 Mouse
- Entrez Gene: 366783 Rat
- Omim: 613306 Human
- SwissProt: Q96BT7 Human
- SwissProt: Q80Y20 Mouse
- Unigene: 503763 Human
- Unigene: 116968 Mouse
see all -
別名
- 4930562C03Rik antibody
- 8030431D03Rik antibody
- 9430088N01Rik antibody
see all
画像
-
Western blot - Anti-ALKBH8 antibody (ab113512)All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : A549 cell lysate
Predicted band size: 75 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH8 antibody (ab113512)ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab113512 は 1 報の論文で使用されています。
- Delaunay S et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). PubMed: 27811057