Anti-ALKBH4 抗体 (ab113500)
Key features and details
- Rabbit polyclonal to ALKBH4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ALKBH4 antibody
ALKBH4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ALKBH4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Recombinant fragment, corresponding to amino acids 38-282 of Human ALKBH4 (BC002820).
-
ポジティブ･コントロール
- Human fetal spleen, fetal liver and fetal kidney lysates; Human fetal pancreas tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).
|
|
| IHC-P |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa). |
|
IHC-P
1/100 - 1/500. |
ターゲット情報
-
機能
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation. -
組織特異性
Widely expressed, with highest expression in pancreas, ovary and spleen. -
配列類似性
Belongs to the alkB family. -
細胞内局在
Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody. - Information by UniProt
-
参照データベース
- Entrez Gene: 54784 Human
- Entrez Gene: 72041 Mouse
- Entrez Gene: 288587 Rat
- Omim: 613302 Human
- SwissProt: Q9NXW9 Human
- SwissProt: Q9D8F1 Mouse
- Unigene: 658598 Human
- Unigene: 486605 Mouse
-
別名
- 2010004B12Rik antibody
- ABH4 antibody
- Abj4 antibody
see all
画像
-
Western blot - Anti-ALKBH4 antibody (ab113500)All lanes : Anti-ALKBH4 antibody (ab113500) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH4 antibody (ab113500)ab113500, at 1/100 dilution, staining ALKBH4 in Formalin-fixed, Paraffin-embedded Human fetal pancreas tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab113500 は論文での使用が確認できていません。