Anti-ALKBH2 抗体 [EPR6177(2)] (ab154859)
![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.co.jp/ps/products/154/ab154859/Images/ab154859-339715-ab154859WB2.jpg "Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6177(2)] to ALKBH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ALKBH2 antibody [EPR6177(2)]
ALKBH2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6177(2)] to ALKBH2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human ALKBH2.
Database link: Q6NS38 -
ポジティブ･コントロール
- Human fetal brain, Human testis, 293T, Mouse brain, Mouse heart, Rat brain, Rat heart Jurkat and HeLa lysates;
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6177(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154859の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
-
機能
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. -
組織特異性
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. -
配列類似性
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain. -
細胞内局在
Nucleus. Detected in replication foci during S-phase. - Information by UniProt
-
参照データベース
- Entrez Gene: 121642 Human
- Entrez Gene: 231642 Mouse
- Entrez Gene: 304578 Rat
- Omim: 610602 Human
- SwissProt: Q6NS38 Human
- SwissProt: Q6P6J4 Mouse
- Unigene: 374458 Human
- Unigene: 332593 Mouse
-
別名
- 2OG Fe(II) oxy DC1 antibody
- 9530023G02 antibody
- ABH 2 antibody
see all
画像
-
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.co.jp/ps/products/154/ab154859/Images/ab154859-339714-ab154859WB1.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.co.jp/ps/products/154/ab154859/Images/ab154859-161487-anti-alkbh2-antibody-epr61772-western-blot.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution
Lanes 1 & 3 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa -
![Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.co.jp/ps/products/154/ab154859/Images/ab154859-5-benefits-of-recombinant-antibodies.png)
Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab154859 は 1 報の論文で使用されています。
- Bonilla B et al. The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions. Elife 10:N/A (2021). PubMed: 34723799