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Anti-Alkaline Phosphatase 抗体 (ab354)

Reviews (2)Q&A (7)References (11)

Key features and details

  • Rabbit polyclonal to Alkaline Phosphatase
  • Suitable for: ELISA, IP, WB
  • Reacts with: Escherichia coli
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Alkaline Phosphatase antibody
    Alkaline Phosphatase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Alkaline Phosphatase

  • 由来種

    Rabbit

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

  • アプリケーション

    適用あり: ELISA, IP, WBmore details

  • 種交差性

    交差種: Escherichia coli
    非交差種: Mammals

  • 免疫原

    Full length protein corresponding to Alkaline Phosphatase. Full length protein (E. coli).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:reconstitute in 2ml of dH2O and split into 100ul Abcam unit

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 特記事項（精製）

    Anti-Alkaline Phosphatase (E. Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab354の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 配列類似性

    Belongs to the alkaline phosphatase family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 別名

    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

参考文献 (11)

ab354 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab354 は 11 報の論文で使用されています。

  • Kim JT  et al. Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient. Int J Mol Sci 24:N/A (2023). PubMed: 36769191
  • Seonwoo H  et al. Reduced graphene oxide-incorporated calcium phosphate cements with pulsed electromagnetic fields for bone regeneration. RSC Adv 12:5557-5570 (2022). PubMed: 35425568
  • Hodgkinson T  et al. The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells. Sci Adv 7:N/A (2021). PubMed: 33637520
  • Wang J  et al. The interaction of SKP2 with p27 enhances the progression and stemness of osteosarcoma. Ann N Y Acad Sci 1490:90-104 (2021). PubMed: 33594717
  • Hemnes AR  et al. BMPR2 dysfunction impairs insulin signaling and glucose homeostasis in cardiomyocytes. Am J Physiol Lung Cell Mol Physiol 318:L429-L441 (2020). PubMed: 31850803
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-9 of 9 Abreviews or Q&A

ELISA abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Recombinant protein (Mach1)
Specification
Mach1
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Indirect
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 26 2010

Western blot abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (Mach1)
Loading amount
0.5 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 19 2010

Question

Please help with the following questions: What is the concentration of the antibody to bacterial alkaline phosphatase? Is this an affinity purified polyclonal? Are there ANY other proteins in the preparation?

Read More
Answer

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG concentrations in tissue culture supernatant will range between 0.5 and 1 mg/ml. If you have any further questions then please get back in touch with us.

Read More

Question

I saw an enquiry dated 7-Feb-2001 asking whether this antibody can detect secreted alk phos (SEAP). At the time, the answer was that you have no reports -- have you heard anything further about this application since?

Read More
Answer

This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species.

Read More

Question

I'm having trouble using this product for ELISA at 2µg/ml. Please let me know the recommended conditions.

Read More
Answer

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

Read More

Question

We want to use alkaline phosphatase antibody to determine ALP releasing in periodontal cells (Osteoblasts, PDL fibroblasts and cementoblasts). Does Alp1-ab354 test ALP expression in bone-like cells by immunohistochemistry?

Read More
Answer

No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tested in IHC.

Read More

Question

I would like to know whether your alkaline phosphatase antibody is a marker of Plasma membrane or not.

Read More
Answer

No, this antibody is not a marker of plasma membrane.

Read More

Question

Do you know if your Alp1 Ab can detect secreted alkaline phosphatase (SEAP)?

Read More
Answer

We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

Read More

Question

We want to use alkaline phosphatse as a marker in E. coli cell fraction experiments. To monitor alk. phosphatase we want to use your antibody. Do you know if alk. phosphatase is expressed under all condition in E. coli (at different levels). Many thanks! Jan-Willem

Read More
Answer

We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

Read More

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