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Alkaline Phosphatase Anti-6X His tag® 抗体 [HIS-1] (ab49746)

Reviews (1)Q&A (1)References (6)

Key features and details

  • Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag®
  • Suitable for: WB, Dot blot, ELISA
  • Reacts with: Species independent
  • Conjugation: Alkaline Phosphatase
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

HRP Unconjugated

製品の概要

  • 製品名

    Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag®

  • 由来種

    Mouse

  • 標識

    Alkaline Phosphatase

  • アプリケーション

    適用あり: WB, Dot blot, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Recombinant poly(Histidine)-tagged fusion protein.

  • ポジティブ･コントロール

    • Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.

  • 特記事項

    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

    The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • 一次抗体 備考

    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

  • ポリ/モノ

    モノクローナル

  • クローン名

    HIS-1

  • アイソタイプ

    IgG2a

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab49746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/2000. Predicted molecular weight: 7 kDa.
Dot blot
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
特記事項
WB
1/2000. Predicted molecular weight: 7 kDa.
Dot blot
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (6)

ab49746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab49746 は 6 報の論文で使用されています。

  • Prasad KVSK  et al. DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity. Cells 12:N/A (2023). PubMed: 37566065
  • Wu Z  et al. Overexpression of fucosyltransferase 8 reverses the inhibitory effect of high-dose dexamethasone on osteogenic response of MC3T3-E1 preosteoblasts. PeerJ 9:e12380 (2021). PubMed: 34966572
  • Yucel B  et al. The copper-responsive ScsC protein of Salmonella promotes intramacrophage survival and interacts with the arginine sensor ArtI. FEBS J 287:3827-3840 (2020). PubMed: 32153092
  • Bottermann M  et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe 25:617-629.e7 (2019). PubMed: 30926239
  • Henche AL  et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). PubMed: 25102813
  • Hussack G  et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . PubMed: 21216961

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase)

 Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell)
Loading amount
10 µg
Specification
neuronal cell
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 02 2009

Question

Inquiry: We are looking into AP conjugated antibodies to detect His-tagged proteins on PIP strips (Echelon). The proteins we use carry the pQE30 Histag (RGS-6xHis). Your anti-6xHis is described to detect tags from pET, pRSET, pTRC. Do you have any information on how it would work with the pQE30 tags? Is it suitable for PIP strips? We plan to use BCIP/NBT detection. What is the detection limit/sensitivity of your AP conjugated antibody with BCIP/NBT? We have not worked with AP cojugated Abs and BCIP/NBT before and therefore appreciate any technical advise you can give us.

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Answer

The 6x His-tag should essentially be the same no matter which vector it is expressed by. The affinity of the antibody is more likely to be affected by the proximity of the His-tag to the recombinant protein (N- or C-terminal or within the reading frame) but as long as the tag is accessible I can see no reason why the antibody would not work.

PIP strips are like a modified dot-blot. This antibody is described as being suitable for dot blots on our website so theoretically it should be suitable for this application.

We have not tested the sensitivity limit of ab49746 with BCIP/NBT but for immunoblotting a minimum working dilution of 1:2,000 is determined using bacteria lysates expressing a recombinant histidine-tagged fusion protein and BCIP/NBT substrate. I would recommend determining the optimal working dilutions by titration.

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