Alkaline Phosphatase Anti-6X His tag® 抗体 [AD1.1.10] (ab81652)
![Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)](https://www.abcam.co.jp/ps/products/81/ab81652/Images/ab81652-457451-alkaline-phosphatase-anti-6x-his-tag-antibody-ad1-1-10-western-blot.jpg "Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)")
Key features and details
- Alkaline Phosphatase Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Alkaline Phosphatase Mouse monoclonal [AD1.1.10] to 6X His tag® -
由来種
Mouse -
標識
Alkaline Phosphatase -
特異性
ab81652 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag®. Polyhistidine (HHHHHH) tagged polypeptide.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris -
Concentration information loading... -
精製度
Size exclusion -
ポリ/モノ
モノクローナル -
クローン名
AD1.1.10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81652の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000.
|
| 特記事項 |
|---|
|
WB
1/5000. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)All lanes : Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652) at 1/10000 dilution
Lane 1 : 60 kDa 6X His-tagged protein at 0.3 µg
Lane 2 : 60 kDa 6X His-tagged protein at 0.1 µg
Lane 3 : 60 kDa 6X His-tagged protein at 0.03 µg
Lane 4 : 60 kDa 6X His-tagged protein at 0.01 µg
Lane 5 : 60 kDa 6X His-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab81652 は論文での使用が確認できていません。